胎儿和新生羔羊大脑中的神经递质及其代谢物。

Neurotransmitters and their metabolites in the brains of fetal and newborn lambs.

作者信息

Richards G E, Gluckman P D, Mannelli S C

机构信息

Department of Pediatrics, University of Texas Medical Branch, Galveston.

出版信息

Life Sci. 1987 Oct 19;41(16):1881-4. doi: 10.1016/0024-3205(87)90738-7.

DOI:10.1016/0024-3205(87)90738-7

PMID:2443785

Abstract

We tested the hypothesis that there is an orderly progressive increase in neurotransmitters in the brains of fetal and neonatal sheep. The pregnant ewes or newborns were killed with an intravenous overdose of pentobarbitone. Brains were removed immediately and frozen at -80 degrees C for later dissection and measurement of norepinephrine (NE), dopamine (DA), serotonin (5HT), homovanillic acid (HVA) and hydroxyindole acetic acid (HIAA). Fetuses were studied at 130-135 days gestation (term gestation 147 days), 140-145 days gestation and 1-5 days after birth. The only compound that was significantly different at the three ages was HIAA. Significant regional differences for NE, DA, and HVA, but not for 5HT were demonstrated.

摘要

我们验证了一个假设，即胎儿和新生绵羊大脑中的神经递质会有有序的逐步增加。给怀孕的母羊或新生羊静脉注射过量戊巴比妥使其死亡。立即取出大脑并在-80摄氏度下冷冻，以便日后解剖并测量去甲肾上腺素（NE）、多巴胺（DA）、血清素（5HT）、高香草酸（HVA）和羟基吲哚乙酸（HIAA）。研究了妊娠130 - 135天（足月妊娠147天）、妊娠140 - 145天以及出生后1 - 5天的胎儿。在这三个年龄段中唯一有显著差异的化合物是HIAA。NE、DA和HVA存在显著的区域差异，但5HT没有。