从人关节软骨中分离蛋白聚糖。
Isolation of proteoglycans from human articular cartilage.
作者信息
Bayliss M T, Ali S Y
出版信息
Biochem J. 1978 Jan 1;169(1):123-32. doi: 10.1042/bj1690123.
Abstract
Proteoglycans were extracted from normal human articular cartilage of various ages with 4M-guanidinium chloride and were purified and characterized by using preformed linear CsCl density gradients. With advancing age, there was a decrease in high-density proteoglycans of low protein/uronic acid weight ratio and an increase in the proportion of lower-density proteoglycans, richer in keratan sulphate and protein. Proteoglycans of each age were also shown to disaggregate in 4M-guanidinium chloride and at low pH and to reaggregate in the presence of hyaluronic acid and/or low-density fractions. Osteoarthrotic-cartilage extracts had an increased content of higher-density proteoglycans compared with normal cartilage of the same age, and results also suggested that these were not mechanical or enzymic degradation products, but were possibly proteoglycans of an immature nature.
摘要
用4M氯化胍从不同年龄正常人关节软骨中提取蛋白聚糖，并用预制线性氯化铯密度梯度进行纯化和表征。随着年龄增长，低蛋白/糖醛酸重量比的高密度蛋白聚糖减少，硫酸角质素和蛋白质含量更高的低密度蛋白聚糖比例增加。每个年龄段的蛋白聚糖在4M氯化胍和低pH条件下也会解聚，并在透明质酸和/或低密度组分存在时重新聚集。与同年龄的正常软骨相比，骨关节炎软骨提取物中高密度蛋白聚糖的含量增加，结果还表明这些不是机械或酶促降解产物，而可能是未成熟性质的蛋白聚糖。
相似文献
1
Isolation of proteoglycans from human articular cartilage.从人关节软骨中分离蛋白聚糖。
Biochem J. 1978 Jan 1;169(1):123-32. doi: 10.1042/bj1690123.
2
Age-related changes in the composition and structure of human articular-cartilage proteoglycans.人类关节软骨蛋白聚糖的组成和结构的年龄相关变化。
Biochem J. 1978 Dec 15;176(3):683-93. doi: 10.1042/bj1760683.
3
Articular-cartilage proteoglycans in aging and osteoarthritis.衰老与骨关节炎中的关节软骨蛋白聚糖
Biochem J. 1978 Jan 1;169(1):143-56. doi: 10.1042/bj1690143.
4
Structure of proteoglycans from different layers of human articular cartilage.来自人类关节软骨不同层的蛋白聚糖结构
Biochem J. 1983 Feb 1;209(2):387-400. doi: 10.1042/bj2090387.
5
Age-related changes in the chemical composition of bovine articular cartilage. The structure of high-density proteoglycans.牛关节软骨化学成分的年龄相关性变化。高密度蛋白聚糖的结构。
Biochem J. 1981 Feb 1;193(2):459-68. doi: 10.1042/bj1930459.
6
The properties of proteoglycan prepared from human articular cartilage by using associative caesium chloride gradients of high and low starting densities.通过使用具有高低起始密度的联合氯化铯梯度从人关节软骨制备的蛋白聚糖的性质。
Biochem J. 1985 Nov 15;232(1):111-7. doi: 10.1042/bj2320111.
7
The inability to prepare high-buoyant-density proteoglycan aggregates from extracts of normal adult human articular cartilage.无法从正常成人关节软骨提取物中制备高浮力密度蛋白聚糖聚集体。
Biochem J. 1984 Aug 1;221(3):637-44. doi: 10.1042/bj2210637.
8
Proteoglycans isolated from dissociative extracts of differently aged human articular cartilage: characterization of naturally occurring hyaluronan-binding fragments of aggrecan.从不同年龄的人关节软骨解离提取物中分离出的蛋白聚糖：聚集蛋白聚糖天然存在的透明质酸结合片段的表征
Biochem J. 1994 Dec 15;304 ( Pt 3)(Pt 3):887-94. doi: 10.1042/bj3040887.
9
Proteoglycans of the intervertebral disc. Absence of degradation during the isolation of proteoglycans from the intervertebral disc.椎间盘的蛋白聚糖。从椎间盘中分离蛋白聚糖时未发生降解。
Biochem J. 1979 Jun 1;179(3):573-8. doi: 10.1042/bj1790573.
10
Extractable proteoglycan from human femoral-head articular cartilage.从人股骨头关节软骨中提取的蛋白聚糖
Biochem J. 1981 Nov 1;199(2):433-9. doi: 10.1042/bj1990433.
引用本文的文献
1
Macromolecular Interactions in Cartilage Extracellular Matrix Vary According to the Cartilage Type and Location.软骨细胞外基质中的大分子相互作用根据软骨类型和位置而变化。
Cartilage. 2021 Dec;13(2_suppl):476S-485S. doi: 10.1177/19476035211000811. Epub 2021 Mar 22.
2
Contaminants in commercial preparations of 'purified' small leucine-rich proteoglycans may distort mechanistic studies.“纯化的”富含亮氨酸小分子蛋白聚糖商业制剂中的污染物可能会扭曲机制研究。
Biosci Rep. 2017 Jan 13;37(1). doi: 10.1042/BSR20160465. Print 2017 Feb 28.
3
Biosynthesis of collagen and other matrix proteins by articular cartilage in experimental osteoarthrosis.实验性骨关节炎中关节软骨对胶原蛋白及其他基质蛋白的生物合成
Biochem J. 1980 Jun 15;188(3):823-37. doi: 10.1042/bj1880823.
4
Extractable proteoglycan from human femoral-head articular cartilage.从人股骨头关节软骨中提取的蛋白聚糖
Biochem J. 1981 Nov 1;199(2):433-9. doi: 10.1042/bj1990433.
5
Proteoglycans in normal and severely osteoarthritic human cartilage.正常及重度骨关节炎人类软骨中的蛋白聚糖
Biochem J. 1980 Jun 1;187(3):781-7. doi: 10.1042/bj1870781.
6
Control of proteoglycan synthesis. Studies on the activation of synthesis observed during culture of articular cartilages.蛋白聚糖合成的调控。关于关节软骨培养过程中观察到的合成激活的研究。
Biochem J. 1980 Apr 15;188(1):119-30. doi: 10.1042/bj1880119.
7
Proteoglycan aggregate formation by articular chondrocytes. Decrease in link-protein synthesis during culture.关节软骨细胞形成蛋白聚糖聚集体。培养过程中连接蛋白合成减少。
Biochem J. 1983 Sep 15;214(3):855-64. doi: 10.1042/bj2140855.
8
Isolation and characterization of high-buoyant-density proteoglycans from bovine femoral-head cartilage.从牛股骨头软骨中分离并鉴定高浮力密度蛋白聚糖
Biochem J. 1983 Aug 1;213(2):355-62. doi: 10.1042/bj2130355.
9
The inability to prepare high-buoyant-density proteoglycan aggregates from extracts of normal adult human articular cartilage.无法从正常成人关节软骨提取物中制备高浮力密度蛋白聚糖聚集体。
Biochem J. 1984 Aug 1;221(3):637-44. doi: 10.1042/bj2210637.
10
Structure of proteoglycans from different layers of human articular cartilage.来自人类关节软骨不同层的蛋白聚糖结构
Biochem J. 1983 Feb 1;209(2):387-400. doi: 10.1042/bj2090387.
本文引用的文献
1
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
2
STUDIES ON A HUMAN THYROID PROTEASE.关于一种人类甲状腺蛋白酶的研究。
J Med Lab Technol. 1964 Jan;21:1-20.
3
ON THE INTERACTION BETWEEN POLYSACCHARIDES AND OTHER MACROMOLECULES. II. THE TRANSPORT OF GLOBULAR PARTICLES THROUGH HYALURONIC ACID SOLUTIONS.论多糖与其他大分子之间的相互作用。II. 球状颗粒在透明质酸溶液中的转运
Biochim Biophys Acta. 1963 Oct 29;78:351-9. doi: 10.1016/0006-3002(63)91645-7.
4
A modified uronic acid carbazole reaction.一种改良的糖醛酸咔唑反应。
Anal Biochem. 1962 Oct;4:330-4. doi: 10.1016/0003-2697(62)90095-7.
5
The effect of hyaluronic acid on the sedimentation rate of other substances.透明质酸对其他物质沉降速率的影响。
Biochim Biophys Acta. 1961 May 13;49:258-64. doi: 10.1016/0006-3002(61)90125-1.
6
A new universal buffer system.一种新型通用缓冲系统。
Nature. 1961 Sep 9;191:1099-100. doi: 10.1038/1911099a0.
7
Ultracentrifugal fractionation of bovine nasal chondromucoprotein.牛鼻软骨粘蛋白的超速离心分级分离
J Biol Chem. 1960 Oct;235:2870-5.
8
The determination of glucosamine and galactosamine.葡萄糖胺和半乳糖胺的测定
Biochem J. 1955 Dec;61(4):586-9. doi: 10.1042/bj0610586.
9
Proteinpolysaccharides from human articular and costal cartilage.来自人体关节软骨和肋软骨的蛋白多糖。
J Clin Invest. 1965 Oct;44(10):1647-56. doi: 10.1172/JCI105271.
10
A quantitative method for estimation of acid polysaccharides in blood.一种测定血液中酸性多糖的定量方法。
Biochim Biophys Acta. 1969 May 6;177(3):641-3.
Zotero 插件