Parrilla Eduardo, Armengot Miguel, Mata Manuel, Sánchez-Vílchez José Manuel, Cortijo Julio, Hueso José L, Riera Jaime, Moratal David
Biomechanics Institute of Valencia (IBV), Spain.
Otorrinolaringology Service and Primary Ciliary Dyskinesia Unit of the Hospital General Universitario and with the Facultat de Medicina, Universitat de València, Valencia, Spain.
Comput Med Imaging Graph. 2014 Apr;38(3):163-70. doi: 10.1016/j.compmedimag.2013.12.010. Epub 2013 Dec 31.
Primary ciliary dyskinesia implies cilia with defective or total absence of motility, which may result in sinusitis, chronic bronchitis, bronchiectasis and male infertility. Diagnosis can be difficult and is based on an abnormal ciliary beat frequency (CBF) and beat pattern. In this paper, we present a method to determine CBF of isolated cells through the analysis of phase-contrast microscopy images, estimating cilia motion by means of an optical flow algorithm. After having analyzed 28 image sequences (14 with a normal beat pattern and 14 with a dyskinetic pattern), the normal group presented a CBF of 5.2 ± 1.6 Hz, while the dyskinetic patients presented a 1.9 ± 0.9 Hz CBF. The cutoff value to classify a dyskinetic specimen was set to 3.45 Hz (sensitivity 0.86, specificity 0.93). The presented methodology has provided excellent results to objectively diagnose PCD.
原发性纤毛运动障碍意味着纤毛存在运动缺陷或完全缺乏运动能力，这可能导致鼻窦炎、慢性支气管炎、支气管扩张和男性不育。诊断可能具有挑战性，其依据是异常的纤毛摆动频率（CBF）和摆动模式。在本文中，我们提出了一种通过分析相差显微镜图像来确定分离细胞的CBF的方法，借助光流算法估计纤毛运动。在分析了28个图像序列（14个具有正常摆动模式和14个具有运动障碍模式）后，正常组的CBF为5.2±1.6Hz，而运动障碍患者的CBF为1.9±0.9Hz。将运动障碍标本分类的临界值设定为3.45Hz（敏感性0.86，特异性0.93）。所提出的方法为客观诊断原发性纤毛运动障碍提供了出色的结果。
