Venkataraman Nagaiyanallur V, Cremmel Clément V M, Zink Christian, Huber Rebecca P, Spencer Nicholas D
Laboratory for Surface Science and Technology, Department of Materials, ETH Zurich, Zurich, Switzerland.
Laboratory for Surface Science and Technology, Department of Materials, ETH Zurich, Zurich, Switzerland; Phonak AG, Stäfa, Switzerland.
Methods Cell Biol. 2014;119:91-121. doi: 10.1016/B978-0-12-416742-1.00006-8.
Surface-chemical and -morphological gradients can be extremely useful in cell-biological research as high-throughput screening tools-for example, exposing a given set of cells to many different surface conditions at once, under identical ambient conditions, in order to monitor cell behavior such as proliferation or specific gene expression. They can also be used to investigate the effects of gradients themselves on cell behavior, such as migration. A number of simple, reliable techniques for both chemical- and morphological-gradient fabrication have been developed in our laboratories and are described in detail in the following.
表面化学梯度和形态学梯度在细胞生物学研究中作为高通量筛选工具可能极其有用，例如，在相同的环境条件下，让给定的一组细胞同时暴露于许多不同的表面条件下，以便监测细胞行为，如增殖或特定基因表达。它们还可用于研究梯度本身对细胞行为的影响，如迁移。我们实验室已经开发了许多用于化学梯度和形态学梯度制备的简单、可靠的技术，并将在下文详细描述。
