AdvanDx Inc., Woburn, MA.
AdvanDx Inc., Woburn, MA.
Diagn Microbiol Infect Dis. 2014 Apr;78(4):338-42. doi: 10.1016/j.diagmicrobio.2013.12.004. Epub 2013 Dec 14.
The performance of a diagnostic method for detection and identification of Enterococcus spp. directly from positive blood culture was evaluated in a clinical study. The method, Enterococcus QuickFISH BC, is a second-generation peptide nucleic acid (PNA) fluorescence in situ hybridization (FISH) test, which uses a simplified, faster assay procedure. The test uses fluorescently labeled PNA probes targeting 16S rRNA to differentiate Enterococcus faecalis from other Enterococcus spp. by the color of the cellular fluorescence. Three hundred fifty-six routine blood culture samples were tested; only 2 discordant results were recorded. The sensitivities for detection of Enterococcus faecalis and non-faecalis Enterococcus were 100% (106/106) and 97.0% (65/67), respectively, and the combined specificity of the assay was 100%. The combined positive and negative predictive values of the assay were 100% (171/171) and 98.9% (185/187), respectively.
我们在一项临床研究中评估了一种直接从阳性血培养物中检测和鉴定肠球菌属的诊断方法的性能。该方法为第二代肽核酸（PNA）荧光原位杂交（FISH）检测技术，即 Enterococcus QuickFISH BC，采用简化、更快的检测程序。该检测使用针对 16S rRNA 的荧光标记 PNA 探针，通过细胞荧光的颜色区分粪肠球菌和其他肠球菌属。我们对 356 份常规血培养样本进行了检测；仅记录了 2 个不一致的结果。检测粪肠球菌和非粪肠球菌肠球菌的灵敏度分别为 100%（106/106）和 97.0%（65/67），该检测的综合特异性为 100%。检测的综合阳性和阴性预测值分别为 100%（171/171）和 98.9%（185/187）。
