CAS Key Laboratory of Tropical Marine Bio-resources and Ecology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, 164 West Xingang Road, Guangzhou 510301, China.
CAS Key Laboratory of Tropical Marine Bio-resources and Ecology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, 164 West Xingang Road, Guangzhou 510301, China.
Gene. 2014 Apr 1;538(2):313-22. doi: 10.1016/j.gene.2014.01.031. Epub 2014 Jan 16.
Differential growth of the pearl oyster Pinctada fucata still exists in the aquaculture production. There is no systematic study of the entire transcriptome of differential gene expression in P. fucata in the literature. In this study, high-throughput Illumina/HiSeq™ 2000 RNA-Seq was used to examine the differences of gene expression in large (L) and small oysters (S). In total, 74,293 and 76,635 unigenes were generated from L and S oysters, respectively. RT quantitative PCR (qPCR) analysis showed that the differential expression pattern of 19 out of 34 selected genes was consistent with the results of RNA-Seq analysis: 14 genes (11 for growth, 1 for reproduction and 2 for shell formation) were expressed more highly in S, 5 genes (1 for growth, 1 for reproduction and 3 for the immune system) were expressed more highly in L; 3 genes associated with the immune system were opposite to it; and no difference was found for the remaining 12 genes. Another 9 shell formation-related genes in L and S were examined by qPCR: 1 gene was expressed more highly in L, 5 genes were expressed more highly in S and no difference was found for the remaining 3 genes. Some genes related to growth and development, shell formation and reproduction were expressed more highly in S compared to L. This phenomenon could be explained by "catch-up growth". The results of this study will help toward a comprehensive understanding of the complexity of differential growth between P. fucata individuals and provide valuable information for future research.
珍珠贝 Pinctada fucata 在水产养殖生产中仍然存在差异生长。文献中尚无关于 P. fucata 差异基因表达的整个转录组的系统研究。在这项研究中，使用高通量 Illumina/HiSeq™ 2000 RNA-Seq 来检查大（L）和小（S）牡蛎之间基因表达的差异。总共从 L 和 S 牡蛎中生成了 74,293 和 76,635 个基因。RT 定量 PCR（qPCR）分析显示，34 个选定基因中有 19 个的差异表达模式与 RNA-Seq 分析结果一致：14 个基因（11 个与生长有关，1 个与繁殖有关，2 个与贝壳形成有关）在 S 中表达更高，5 个基因（1 个与生长有关，1 个与繁殖有关，3 个与免疫系统有关）在 L 中表达更高；3 个与免疫系统相关的基因与之相反；其余 12 个基因没有差异。另外还通过 qPCR 检查了 L 和 S 中的 9 个贝壳形成相关基因：1 个基因在 L 中表达更高，5 个基因在 S 中表达更高，其余 3 个基因没有差异。与生长发育、贝壳形成和繁殖相关的一些基因在 S 中的表达高于 L。这种现象可以用“追赶生长”来解释。本研究的结果将有助于全面了解 P. fucata 个体之间差异生长的复杂性，并为未来的研究提供有价值的信息。
