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Molecular hybridization with RNA probes in concentrated solutions of guanidine thiocyanate.

作者信息

Thompson J, Gillespie D

机构信息

Department of Neoplastic Diseases, Hahnemann University, Philadelphia, Pennsylvania 19102.

出版信息

Anal Biochem. 1987 Jun;163(2):281-91. doi: 10.1016/0003-2697(87)90225-9.

DOI:10.1016/0003-2697(87)90225-9
PMID:2444134
Abstract

Hybridizations with RNA probes were performed in 3-6 M guanidine thiocyanate concentrations where cells can be solubilized. At these concentrations the melting temperature of hybrids and the optimum temperature of hybrid formation are greatly reduced. The rate of hybridization in GuSCN at room temperature was greater than that in 50% formamide at 42 degrees C. Hybridizations were performed on DNA and RNA in cells dissolved in GuSCN without prior nucleic acid purification.

摘要

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