Pedlar J
Department of Oral and Maxillo-facial Surgery, University of Manchester Dental School, England, U.K.
Arch Oral Biol. 1987;32(3):195-9. doi: 10.1016/0003-9969(87)90134-8.
Eighteen specimens of palatal mucosa were taken from 17 human subjects. Paraffin-wax sections were stained by routine methods and with various techniques to demonstrate glycosaminoglycans (GAG). In some sections, GAG were removed by selective degradative procedures before staining. Beneath all rugae, there were myxoid areas varying in size and marginal definition. Collagen fibres were few; elastic and reticulin fibres were numerous in a minority of sections. Alcianophilia at pH 2.5, preventable by streptomyces hyaluronidase digestion, suggested the presence of hyaluronic acid beneath the rugae. Alcian-blue staining at pH 1.0 and with the critical electrolyte concentration method using 0.5 M MgCl2 did not distinguish the myxoid tissue from the surrounding connective tissue and could be prevented by digestion with testicular hyaluronidase or chondroitinase ABC. Chondroitin sulphate and, or dermatan sulphate thus may be present but were not localized to the myxoid tissue. This unusual zone of loose connective tissue may act as a physical buffer resisting the local effects of high loads by allowing reversible extrusion of the water.
从17名人类受试者身上采集了18个腭黏膜标本。石蜡切片采用常规方法及各种技术进行染色，以显示糖胺聚糖（GAG）。在一些切片中，GAG在染色前通过选择性降解程序去除。在所有皱襞下方，均存在大小和边缘清晰度各异的黏液样区域。胶原纤维较少；在少数切片中弹性纤维和网状纤维较多。pH值为2.5时的阿利新蓝染色，可被链霉菌透明质酸酶消化所阻断，提示皱襞下方存在透明质酸。pH值为1.0时的阿尔辛蓝染色以及使用0.5M氯化镁的临界电解质浓度法，无法区分黏液样组织与周围结缔组织，可通过睾丸透明质酸酶或软骨素酶ABC消化来阻断。因此可能存在硫酸软骨素和/或硫酸皮肤素，但并非局限于黏液样组织。这个不寻常的疏松结缔组织区域可能起到物理缓冲作用，通过允许水的可逆挤出抵抗高负荷的局部影响。
