Ningbo Institute of Materials Technology and Engineering, Chinese Academy of Sciences, Ningbo, 315201, China.
Anal Bioanal Chem. 2014 Mar;406(7):1903-8. doi: 10.1007/s00216-013-7591-9. Epub 2014 Jan 18.
A simple, inexpensive yet highly selective colorimetric indicator-displacement assay array for the simultaneous detection and identification of three important biothiols at micromolar concentrations under physiological conditions and in real samples has been developed in this work. With use of an array composed of metal indicators and metal ions, clear differentiation among cysteine, homocysteine and glutathione was achieved. On the basis of the colour change of the array, quantification of each analyte was accomplished easily, and different biothiols were identified readily using standard chemometric approaches (hierarchical clustering analysis). Moreover, the colorimetric sensor array was not responsive to changes with 19 other natural amino acids, and it showed excellent reproducibility. Importantly, the sensor array developed was successfully applied to the determination and identification of the three biothiols in a real biological sample.
本工作开发了一种简单、廉价但高度选择性的比色指示剂置换分析阵列，可在生理条件下和实际样品中同时检测和识别三种重要的生物硫醇，浓度可达微摩尔级。该阵列由金属指示剂和金属离子组成，可实现半胱氨酸、同型半胱氨酸和谷胱甘肽的明显区分。基于阵列的颜色变化，很容易完成每种分析物的定量，并且可以使用标准化学计量学方法（层次聚类分析）轻松识别不同的生物硫醇。此外，比色传感器阵列对 19 种其他天然氨基酸的变化没有响应，并且具有出色的重现性。重要的是，开发的传感器阵列成功应用于实际生物样品中三种生物硫醇的测定和识别。
