大鼠肝脏参与聚合免疫球蛋白A转胞吞作用和去唾液酸胎球蛋白内吞作用的内吞区室的快速亚细胞分级分离。
Rapid subcellular fractionation of the rat liver endocytic compartments involved in transcytosis of polymeric immunoglobulin A and endocytosis of asialofetuin.
作者信息
Branch W J, Mullock B M, Luzio J P
机构信息
Department of Clinical Biochemistry, University of Cambridge, Addenbrooke's Hospital, U.K.
出版信息
Biochem J. 1987 Jun 1;244(2):311-5. doi: 10.1042/bj2440311.
Abstract
The distributions of two endocytosed radiolabelled ligands (polymeric immunoglobulin A and asialofetuin) in rat liver endocytic compartments were investigated by using rapid subcellular fractionation of post-mitochondrial supernatants on vertical density gradients of Ficoll or Nycodenz. Two endocytic compartments were identified, both ligands being initially associated with a light endocytic-vesicle fraction on Ficoll gradients, asialofetuin then accumulating in denser endosomes before transfer to lysosomes for degradation.
摘要
通过对线粒体后上清液在菲可（Ficoll）或 Nycodenz 的垂直密度梯度上进行快速亚细胞分级分离，研究了两种内吞的放射性标记配体（聚合免疫球蛋白 A 和去唾液酸胎球蛋白）在大鼠肝脏内吞区室中的分布。确定了两个内吞区室，两种配体最初都与菲可梯度上的轻内吞小泡部分相关联，去唾液酸胎球蛋白随后在密度更高的内体中积累，然后转移到溶酶体中进行降解。
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