Branch W J, Mullock B M, Luzio J P
Department of Clinical Biochemistry, University of Cambridge, Addenbrooke's Hospital, U.K.
Biochem J. 1987 Jun 1;244(2):311-5. doi: 10.1042/bj2440311.
The distributions of two endocytosed radiolabelled ligands (polymeric immunoglobulin A and asialofetuin) in rat liver endocytic compartments were investigated by using rapid subcellular fractionation of post-mitochondrial supernatants on vertical density gradients of Ficoll or Nycodenz. Two endocytic compartments were identified, both ligands being initially associated with a light endocytic-vesicle fraction on Ficoll gradients, asialofetuin then accumulating in denser endosomes before transfer to lysosomes for degradation.
通过对线粒体后上清液在菲可（Ficoll）或 Nycodenz 的垂直密度梯度上进行快速亚细胞分级分离，研究了两种内吞的放射性标记配体（聚合免疫球蛋白 A 和去唾液酸胎球蛋白）在大鼠肝脏内吞区室中的分布。确定了两个内吞区室，两种配体最初都与菲可梯度上的轻内吞小泡部分相关联，去唾液酸胎球蛋白随后在密度更高的内体中积累，然后转移到溶酶体中进行降解。
