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[氯化钠和脱落酸处理对珍珠粟幼苗蛋白质代谢及氨基酸代谢其他一些酶的影响]

[Influence of NaCl- and abscisic acid treatment on protein metabolism and some further enzymes of amino acid metabolism in seedlings of Pennisetum typhoides].

作者信息

Huber W

机构信息

Institut für Botanik der Technischen Universität München, Arcisstr. 21, D-8, München 2, Federal Republic of Germany.

出版信息

Planta. 1974 Jan;121(3):225-35. doi: 10.1007/BF00389323.

Abstract

The effects of sodium chloride (NaCl) and abscisic acid (ABA) on protein synthesis, protein hydrolysis, glutamine synthetase, Δ-pyrroline-5-carboxylate reductase and amino acid composition have been studied in aerial parts of Pennisetum typhoides (Stapf et Hubbard) seedlings. Whereas protein synthesis is inhibited by NaCl and ABA, the activity or the synthesis of leucine-arylamidase, glutamine synthetase and Δ-pyrroline-5-carboxylate reductase is promoted by both the substances. The results of the amino acid analysis showed that in the presence of NaCl and ABA the amounts of alanine, aspartate and ammonia decreased, while the concentrations of glutamate, and especially that of proline increased greatly. The possible significance of the results in explaining the proline accumulation under stress conditions and ABA-treatment is discussed.

摘要

研究了氯化钠(NaCl)和脱落酸(ABA)对狼尾草(Stapf et Hubbard)幼苗地上部分蛋白质合成、蛋白质水解、谷氨酰胺合成酶、Δ-吡咯啉-5-羧酸还原酶及氨基酸组成的影响。虽然蛋白质合成受到NaCl和ABA的抑制,但亮氨酸芳基酰胺酶、谷氨酰胺合成酶和Δ-吡咯啉-5-羧酸还原酶的活性或合成受到这两种物质的促进。氨基酸分析结果表明,在NaCl和ABA存在的情况下,丙氨酸、天冬氨酸和氨的含量下降,而谷氨酸,尤其是脯氨酸的浓度大幅增加。讨论了这些结果在解释胁迫条件下和ABA处理下脯氨酸积累的可能意义。

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