Hickey W F, Cohen J A, Burns J B
Department of Pathology, School of Medicine, University of Pennsylvania, Philadelphia 19104.
Cell Immunol. 1987 Oct 15;109(2):272-81. doi: 10.1016/0008-8749(87)90311-x.
A quantitative immunohistochemical comparison of actively and adoptively induced experimental allergic encephalomyelitis (EAE) in the Lewis rat was performed. Since the methods of EAE induction of these two systems and the kinetics of disease appearance are different, while the histopathology, disease manifestations, duration, and severity are similar, this study sought to identify any differences which exist at the level of the target organ. The number of cells expressing the T helper (W3/25) or suppressor/cytotoxic (OX-8) phenotypes and the number of Ia-positive cells found in the spinal cord of animals given EAE by one of the two methods were compared at two time points at which maximal similarities should exist. The results show that during acute adoptively induced EAE the inflammatory infiltrate contains a larger number of T helper (TH) cells per unit area than in acute active EAE. With the resolution of clinical signs of EAE, the disappearance of cells from the spinal cord is more rapid in adoptive EAE. In contrast, the inflammatory infiltrate and Ia-positive parenchymal cells persist in active EAE following recovery. These results suggest that actively and adoptively induced EAE may differ with respect to the effector mechanisms and/or the mechanisms of recovery at the level of the target organ.
对Lewis大鼠主动诱导和过继诱导的实验性变态反应性脑脊髓炎（EAE）进行了定量免疫组织化学比较。由于这两种诱导EAE的方法以及疾病出现的动力学不同，而组织病理学、疾病表现、持续时间和严重程度相似，本研究旨在确定在靶器官水平上存在的任何差异。在两个应存在最大相似性的时间点，比较了通过两种方法之一给予EAE的动物脊髓中表达辅助性T细胞（W3/25）或抑制性/细胞毒性T细胞（OX-8）表型的细胞数量以及Ia阳性细胞数量。结果显示，在急性过继诱导的EAE中，每单位面积的炎症浸润中辅助性T细胞（TH）数量比急性主动诱导的EAE中更多。随着EAE临床症状的消退，过继诱导的EAE中脊髓细胞的消失更快。相反，主动诱导的EAE恢复后，炎症浸润和Ia阳性实质细胞持续存在。这些结果表明，主动诱导和过继诱导的EAE在靶器官水平上的效应机制和/或恢复机制可能存在差异。
