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核心技术专利：CN118964589B侵权必究

核心技术专利：CN118964589B侵权必究

Department of Parasitology, Juntendo University School of Medicine, Tokyo, Japan.

Comp Biochem Physiol B. 1987;87(4):655-8. doi: 10.1016/0305-0491(87)90369-5.

At the lowered concentrations of 0.5 mM ATP and 1.5 mM MgCl2, 2.0 mM UTP, UDP and UMP inhibited the activity of Crithidia fasciculata carbamoyl-phosphate synthetase II by about 65, 80 and 40% respectively. 2. The result suggests that feedback inhibition of the activity by uridine nucleotides is a mechanism of regulation of the de novo pyrimidine biosynthetic pathway in C. fasciculata. 3. ADP, AMP and CDP inhibited the activity (about 70, 40 and 40%). 4. Excess Mg2+ at around 1 mM, relative to the ATP concentration, was required for the maximum activity. 5. 5-Phosphoribosyl 1-pyrophosphate had no significant effect on the activity under various conditions examined.

在ATP浓度降至0.5 mM、MgCl₂浓度降至1.5 mM时，2.0 mM的UTP、UDP和UMP分别使纤细短膜虫氨甲酰磷酸合成酶II的活性抑制约65%、80%和40%。2. 该结果表明，尿苷酸对该活性的反馈抑制是纤细短膜虫嘧啶从头生物合成途径的一种调节机制。3. ADP、AMP和CDP抑制该活性（约70%、40%和40%）。4. 相对于ATP浓度，约1 mM的过量Mg²⁺是最大活性所必需的。5. 在各种检测条件下，5-磷酸核糖-1-焦磷酸对该活性无显著影响。

Department of Parasitology, Juntendo University School of Medicine, Tokyo, Japan.

Comp Biochem Physiol B. 1987;87(4):655-8. doi: 10.1016/0305-0491(87)90369-5.

At the lowered concentrations of 0.5 mM ATP and 1.5 mM MgCl2, 2.0 mM UTP, UDP and UMP inhibited the activity of Crithidia fasciculata carbamoyl-phosphate synthetase II by about 65, 80 and 40% respectively. 2. The result suggests that feedback inhibition of the activity by uridine nucleotides is a mechanism of regulation of the de novo pyrimidine biosynthetic pathway in C. fasciculata. 3. ADP, AMP and CDP inhibited the activity (about 70, 40 and 40%). 4. Excess Mg2+ at around 1 mM, relative to the ATP concentration, was required for the maximum activity. 5. 5-Phosphoribosyl 1-pyrophosphate had no significant effect on the activity under various conditions examined.

在ATP浓度降至0.5 mM、MgCl₂浓度降至1.5 mM时，2.0 mM的UTP、UDP和UMP分别使纤细短膜虫氨甲酰磷酸合成酶II的活性抑制约65%、80%和40%。2. 该结果表明，尿苷酸对该活性的反馈抑制是纤细短膜虫嘧啶从头生物合成途径的一种调节机制。3. ADP、AMP和CDP抑制该活性（约70%、40%和40%）。4. 相对于ATP浓度，约1 mM的过量Mg²⁺是最大活性所必需的。5. 在各种检测条件下，5-磷酸核糖-1-焦磷酸对该活性无显著影响。