Zhou Su-ying, Feng Guo-fei, Chen Guo-rong, Pan Dan, Zhang Pin-nan, Yang Xiao-min, Xia Zuo-li
The Third Clinical Collage, Wenzhou Medical University, Wenzhou 325000, China.
325000 Wenzhou, Department of Pathology, The First Affiliated Hospital of Wenzhou Medical University. Email:
Zhonghua Fu Chan Ke Za Zhi. 2013 Nov;48(11):843-6.
To elucidate the protein expression and gene expression status and the relationship between epidermal growth factor receptor (EGFR) protein expression and EGFR gene status.
Tissue microarray containing 72 cervical squamous cell carcinoma tissues was constructed, and EGFR protein expression and gene status were evaluated by immunohistochemical and fluorescence in situ hybridization (FISH) techniques.
Protein expression of EGFR: 69 of 72 cervical squamous cell carcinomas were observed. The results demonstrated it was significant association with invasion depth, lymph node metastasis and lymph-vessel invasion (χ(2) = 4.998, P < 0.05; χ(2) = 4.299, P < 0.05; χ(2) = 4.686, P < 0.05) in cervical squamous cell carcinomas. For FISH assessing EGFR gene, 64 of 72 carcinomas were observed; 7 of 64 cases showed EGFR gene amplification, and 25 disomy, 23 trisomy and 9 polysomy were detected. There were high levels of protein expression in all the EGFR gene amplification cases, and there were significant association between EGFR protein expression and the gene copy number (χ(2) = 13.564, P < 0.05).
EGFR may participate in the occurrence, progression and metastasis of cervical squamous cell carcinoma. Overexpression of EGFR protein may result from gene amplification and gene copy number increases, which showed that EGFR gene expression status may be a more effective biological indicator of cervical squamous cell carcinoma targeted therapy.
阐明表皮生长因子受体（EGFR）蛋白表达和基因表达状态以及EGFR蛋白表达与EGFR基因状态之间的关系。
构建包含72例宫颈鳞状细胞癌组织的组织芯片，采用免疫组织化学和荧光原位杂交（FISH）技术评估EGFR蛋白表达和基因状态。
EGFR蛋白表达：观察了72例宫颈鳞状细胞癌中的69例。结果表明，其与宫颈鳞状细胞癌的浸润深度、淋巴结转移和淋巴管浸润有显著相关性（χ² = 4.998，P < 0.05；χ² = 4.299，P < 0.05；χ² = 4.686，P < 0.05）。对于评估EGFR基因的FISH，观察了72例癌中的64例；64例中有7例显示EGFR基因扩增，检测到25例二倍体、23例三体和9例多体。所有EGFR基因扩增病例均有高水平的蛋白表达，且EGFR蛋白表达与基因拷贝数之间有显著相关性（χ² = 13.564，P < 0.05）。
EGFR可能参与宫颈鳞状细胞癌的发生、发展和转移。EGFR蛋白的过表达可能源于基因扩增和基因拷贝数增加，这表明EGFR基因表达状态可能是宫颈鳞状细胞癌靶向治疗更有效的生物学指标。
