Ge Hong-shan, Zhang Fan, Li Xiao-he, Chen Hua, Xi Hai-tao, Huang Jian-ying, Zhu Chun-fang, Lü Jie-qiang
Reproductive Medical Center, Second Affiliated Hospital of Wenzhou Medical University, Wenzhou 325000, China. Email:
Reproductive Medical Center, Second Affiliated Hospital of Wenzhou Medical University, Wenzhou 325000, China.
Zhonghua Fu Chan Ke Za Zhi. 2013 Nov;48(11):858-61.
To investigate the effect of whether controlled ovarian hyperstimulation (COH) on mitochondrial DNA (mtDNA) copy number, mitochondrial function, distribution, the level of reactive oxygen species (ROS) in oocytes and the mechanism of oocyte loss in COH.
Matured murine oocytes were classified into COH group and natural cycles (NC) group. The copies of mtDNA, the magnitude of mitochondrial membrane potential (Δφm) and oocyte adenosine triphosphate (ATP) content, pattern of mitochondrial distribution, and ROS levels were evaluated by realtime PCR, immunofluorescence and fluorescence-luciferase mensuration.
The copies of mtDNA, the levels of Δφm, and ATP content in oocytes between COH and NC groups showed statistical difference [(1.15 ± 0.01)×10(5), 0.34 ± 0.03 and (241 ± 20) fmol/oocyte (COH)] versus [(2.15 ± 0.19)×10(5), 0.82 ± 0.07 and (325 ± 11) fmol/oocyte (NC)], respectively(P < 0.05). However, there were no significant differences in the rate of evenly distributed mitochondria and the level of ROS in oocytes from COH and NC [(76.5% (78/102) in COH versus 82.1% (69/84)]; 1.07 ± 0.07 in COH versus 0.93 ± 0.08 in NC (P > 0.05).
It was indicated that non-physiological COH treatments inhibit mtDNA replication, alter mitochondrial function, which might partly be involved in the low development potential of COH oocyte.
探讨控制性卵巢刺激（COH）对线粒体DNA（mtDNA）拷贝数、线粒体功能、分布、卵母细胞中活性氧（ROS）水平的影响以及COH中卵母细胞丢失的机制。
将成熟的小鼠卵母细胞分为COH组和自然周期（NC）组。通过实时聚合酶链反应、免疫荧光和荧光素酶测定法评估mtDNA拷贝数、线粒体膜电位（Δφm）大小、卵母细胞三磷酸腺苷（ATP）含量、线粒体分布模式和ROS水平。
COH组和NC组卵母细胞中mtDNA拷贝数、Δφm水平和ATP含量存在统计学差异[（1.15±0.01）×10⁵、0.34±0.03和（241±20）fmol/卵母细胞（COH）]与[（2.15±0.19）×10⁵、0.82±0.07和（325±11）fmol/卵母细胞（NC）]，分别（P<0.05）。然而，COH组和NC组卵母细胞中线粒体均匀分布率和ROS水平无显著差异[COH组为76.5%（78/102）对82.1%（69/84）]；COH组为1.07±0.07对NC组为0.93±0.08（P>0.05）。
表明非生理性COH处理抑制mtDNA复制，改变线粒体功能，这可能部分参与了COH卵母细胞发育潜能低下的过程。
