Johns D W, Carey R M, Gomez R A, Lynch K, Inagami T, Saye J, Geary K, Farnsworth D E, Peach M J
Department of Internal Medicine, University of Virginia School of Medicine, Charlottesville 22908.
Hypertension. 1987 Nov;10(5):488-96. doi: 10.1161/01.hyp.10.5.488.
Enzymatic dispersion and density gradient (Percoll) sedimentation were used to isolate a population of renin-containing, granule-laden cells (density 1.067 g/ml) from rat kidney cortex. Using immunohistochemistry (light microscopy) and electron microscopy, we defined the presence and ability of these cells to store renin protein(s). A 1000 base pair rat renin complementary DNA was used to show that these cells express the renin gene. The reverse hemolytic plaque assay defined the functional properties of the renin-containing cell. The data are consistent with the postulated inverse relationship between calcium concentration and release of renin. Thus, we have isolated a population of functional rat kidney cells that synthesize, store, and release renin.
采用酶消化法和密度梯度（Percoll）沉降法从大鼠肾皮质中分离出一群含有肾素、充满颗粒的细胞（密度为1.067 g/ml）。运用免疫组织化学（光学显微镜）和电子显微镜技术，我们确定了这些细胞中肾素蛋白的存在及其储存能力。使用一段1000个碱基对的大鼠肾素互补DNA来证明这些细胞表达肾素基因。反向溶血空斑试验确定了含肾素细胞的功能特性。这些数据与钙浓度和肾素释放之间假定的反比关系相一致。因此，我们分离出了一群能够合成、储存和释放肾素的功能性大鼠肾细胞。
