Christiansen G, Andersen H, Birkelund S, Freundt E A
Institute of Medical Microbiology, University of Aarhus, Denmark.
Isr J Med Sci. 1987 Jun;23(6):595-602.
DNAs from 14 strains of Mycoplasma hominis isolated from various habitats, including strain PG21, were analyzed for genomic heterogeneity. DNA-DNA filter hybridization values were from 51 to 91%. Restriction endonuclease digestion patterns, analyzed by agarose gel electrophoresis, revealed no identity or cluster formation between strains. Variation within M. hominis rRNA genes was analyzed by Southern hybridization of EcoRI-cleaved DNA hybridized with a cloned fragment of the rRNA gene from the mycoplasma strain PG50. Five of the M. hominis strains showed identical hybridization patterns. These hybridization patterns were compared with those of 12 other mycoplasma species, which showed a much more complex band pattern. Cloned nonribosomal RNA gene fragments of M. hominis PG21 DNA were analyzed, and the fragments were used to demonstrate heterogeneity among the strains. A monoclonal antibody against strain PG21 was produced. The possible presence of the corresponding antigenic epitope in the other 13 M. hominis strains was analyzed by immunoblotting and compared with results of immunoblotting using polyclonal antibodies against PG21.
对从包括PG21菌株在内的各种生境中分离出的14株人型支原体的DNA进行了基因组异质性分析。DNA - DNA滤膜杂交值在51%至91%之间。通过琼脂糖凝胶电泳分析的限制性内切酶消化模式显示，各菌株之间不存在一致性或聚类形成。通过用来自支原体菌株PG50的rRNA基因的克隆片段与经EcoRI切割的DNA进行Southern杂交，分析了人型支原体rRNA基因内的变异。5株人型支原体菌株显示出相同的杂交模式。将这些杂交模式与其他12种支原体的杂交模式进行比较，后者显示出更为复杂的条带模式。对人型支原体PG21 DNA克隆的非核糖体RNA基因片段进行了分析，这些片段被用于证明菌株间的异质性。制备了针对PG21菌株的单克隆抗体。通过免疫印迹分析了其他13株人型支原体菌株中相应抗原表位的可能存在情况，并与使用针对PG21的多克隆抗体进行免疫印迹的结果进行了比较。
