Blanchard A, Yáñez A, Dybvig K, Watson H L, Griffiths G, Cassell G H
Department of Microbiology, University of Alabama, Birmingham 35294.
J Clin Microbiol. 1993 May;31(5):1358-61. doi: 10.1128/jcm.31.5.1358-1361.1993.
Mycoplasma hominis is a heterogeneous species with DNA-DNA hybridization values ranging from 51 to 100%. We report here the sequencing of the 16S rRNA gene of a strain (183) that greatly differs from the type strain (PG21) of this species. Comparison of 16S rDNA sequences from these two strains showed limited differences, indicating that the two strains belong to the same rRNA species complex. Using these nucleotide sequence data, we established a rapid method for the detection of M. hominis by using polymerase chain reaction. This method was shown to be sensitive and specific when tested with reference strains and clinical isolates.
人型支原体是一种异质菌种，其DNA-DNA杂交值在51%至100%之间。我们在此报告一株菌株（183）的16S rRNA基因测序结果，该菌株与该菌种的模式菌株（PG21）有很大差异。对这两种菌株的16S rDNA序列进行比较，结果显示差异有限，表明这两种菌株属于同一rRNA菌种复合体。利用这些核苷酸序列数据，我们建立了一种通过聚合酶链反应检测人型支原体的快速方法。用参考菌株和临床分离株进行测试时，该方法显示出灵敏性和特异性。
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