Sui W, Cao C, Che W, Chen J, Xue W, Liu P, Guo L, Dai Y
Nephrology Department of Guilin 181st Hospital, Guangxi Key Laboratory of Metabolic Diseases Research, Guilin, Guangxi, China.
College of Life Science, Guangxi Normal University, Guilin, Guangxi, China.
Genet Mol Res. 2014 Mar 17;13(1):1697-706. doi: 10.4238/2014.January.14.5.
The global features of trimethylations of histone 3 at lysine 9 (H3K9me3) have been well studied in recent years; however, most of these studies were performed in mammalian cell lines. In this study, we generated genome-wide maps of H3K9me3 of the human heart and spleen using chromatin immunoprecipitation followed by high-throughput sequencing (ChIP-seq) technology. We examined the global patterns of H3K9me3 in both tissues and found that modifications were closely associated with tissue-specific expression, function, and development. In addition, we found that 169 genes displayed significant H3K9me3 differences between the heart and spleen. Among these genes, 64 were heart-H3K9me3-specific, 87 genes were spleen-H3K9me3-specific, and 18 were shared in both heart- and spleen-H3K9me3. In conclusion, we observed significant differences in H3K9me3 in the heart and spleen, which may help to explain epigenetic differences between these tissues. Such novel findings highlight the significance of H3K9me3 as a potential biomarker or promising target for epigenetic-based disease treatment.
近年来，组蛋白H3赖氨酸9位点三甲基化（H3K9me3）的整体特征已得到充分研究；然而，这些研究大多是在哺乳动物细胞系中进行的。在本研究中，我们利用染色质免疫沉淀结合高通量测序（ChIP-seq）技术绘制了人类心脏和脾脏H3K9me3的全基因组图谱。我们研究了这两种组织中H3K9me3的整体模式，发现这些修饰与组织特异性表达、功能及发育密切相关。此外，我们发现有169个基因在心脏和脾脏之间表现出显著的H3K9me3差异。在这些基因中，64个是心脏特异性H3K9me3基因，87个是脾脏特异性H3K9me3基因，18个在心脏和脾脏的H3K9me3中均有出现。总之，我们观察到心脏和脾脏中H3K9me3存在显著差异，这可能有助于解释这些组织之间的表观遗传差异。这些新发现凸显了H3K9me3作为潜在生物标志物或基于表观遗传学的疾病治疗的有前景靶点的重要性。
