Gorse G J, Kopp W C
Department of Medicine, Huntington Veterans Administration Medical Center, WV.
J Lab Clin Med. 1987 Nov;110(5):592-601.
The response to influenza A virus of peripheral blood mononuclear cells (PBMC) isolated from normal healthy adults was studied by measuring antigen-specific lymphocyte proliferation and interferon and interleukin-2 production by these cells in vitro. Under various experimental conditions, methylprednisolone, doxorubicin, vincristine, and vinblastine each reduced lymphocyte proliferation of the PBMC exposed to influenza A virus antigen. Treatment of cells with these drugs did not reduce expression of influenza A virus antigens by PBMC as detected by flow cytometry and did not reduce cell viability. In vitro treatment of the cells with methylprednisolone and doxorubicin reduced interferon and interleukin-2 yields. Experimental conditions necessary to reduce lymphokine yields varied with the immunosuppressive drug tested. Vincristine and vinblastine treatment of the cells did not reduce interferon or interleukin-2 yields. We conclude that methylprednisolone and doxorubicin at clinically achievable concentrations significantly affected the response of PBMC to influenza A virus in vitro. The effects of these immunosuppressive agents on lymphokine production were different from those reported in previous studies in which mitogens and Newcastle disease virus were used as inducers. The sensitivity of PBMC to these immunosuppressive agents is clearly related to the inducing agent used.
通过体外测量从正常健康成年人分离的外周血单核细胞（PBMC）的抗原特异性淋巴细胞增殖以及这些细胞产生的干扰素和白细胞介素-2，研究了PBMC对甲型流感病毒的反应。在各种实验条件下，甲基强的松龙、阿霉素、长春新碱和长春花碱均降低了暴露于甲型流感病毒抗原的PBMC的淋巴细胞增殖。用这些药物处理细胞，通过流式细胞术检测未降低PBMC中甲型流感病毒抗原的表达，也未降低细胞活力。用甲基强的松龙和阿霉素体外处理细胞降低了干扰素和白细胞介素-2的产量。降低淋巴因子产量所需的实验条件因所测试的免疫抑制药物而异。用长春新碱和长春花碱处理细胞未降低干扰素或白细胞介素-2的产量。我们得出结论，临床上可达到的浓度的甲基强的松龙和阿霉素在体外显著影响PBMC对甲型流感病毒的反应。这些免疫抑制剂对淋巴因子产生的影响与先前使用有丝分裂原和新城疫病毒作为诱导剂的研究中报道的不同。PBMC对这些免疫抑制剂的敏感性显然与所使用的诱导剂有关。
