从虎杖根中分离得到的乙酸乙酯部位及其主要成分大黄素对 EBV 裂解周期的抑制作用。
Inhibition of Epstein-Barr virus lytic cycle by an ethyl acetate subfraction separated from Polygonum cuspidatum root and its major component, emodin.
机构信息
Department of Otolaryngology, Chi Mei Medical Center, Liouying, Tainan 717, Taiwan.
出版信息
Molecules. 2014 Jan 20;19(1):1258-72. doi: 10.3390/molecules19011258.
Polygonum cuspidatum is widely used as a medicinal herb in Asia. In this study, we examined the ethyl acetate subfraction F3 obtained from P. cuspidatum root and its major component, emodin, for their capacity to inhibit the Epstein-Barr virus (EBV) lytic cycle. The cell viability was determined by the MTT [3-(4,5-dimethyldiazol-2-yl)-2,5-diphenyltetrazolium bromide] method. The expression of EBV lytic proteins was analyzed by immunoblot, indirect immunofluorescence and flow cytometric assays. Real-time quantitative PCR was used to assess the EBV DNA replication and the transcription of lytic genes, including BRLF1 and BZLF1. Results showed that the F3 and its major component emodin inhibit the transcription of EBV immediate early genes, the expression of EBV lytic proteins, including Rta, Zta, and EA-D and reduces EBV DNA replication, showing that F3 and emodin are potentially useful as an anti-EBV drug.
虎杖在亚洲被广泛用作药用植物。在这项研究中，我们研究了从虎杖根部提取的乙酸乙酯分馏物 F3 及其主要成分大黄素抑制 EBV 裂解周期的能力。通过 MTT [3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四氮唑溴盐]法测定细胞活力。通过免疫印迹、间接免疫荧光和流式细胞术分析 EBV 裂解蛋白的表达。实时定量 PCR 用于评估 EBV DNA 复制和裂解基因的转录，包括 BRLF1 和 BZLF1。结果表明，F3 和其主要成分大黄素抑制 EBV 早期基因的转录、EBV 裂解蛋白的表达，包括 Rta、Zta 和 EA-D，并减少 EBV DNA 复制，表明 F3 和大黄素可能是一种有用的抗 EBV 药物。
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