Vellenga E, Ostapovicz D, O'Rourke B, Griffin J D
Division of Tumor Immunology, Dana-Farber Cancer Institute, Boston, MA 02115.
Leukemia. 1987 Aug;1(8):584-9.
To further define the growth factors required for the in vitro proliferation of acute myeloblastic leukemic (AML) cells, we have compared the ability of recombinant interleukin-3 (IL-3), granulocyte-macrophage colony stimulating factor (GM-CSF), and granulocyte colony stimulating factor (G-CSF) to support growth of AML colony forming cells (AML-CFU). IL-3, GM-CSF, and G-CSF are active as single growth factors in short-term colony cultures and have additive effects when used in combination in some cases. The effects of these CSFs on the proliferation of AML cells in long-term-cell-suspension cultures were also investigated. These cultures provide an estimate of the "self-renewal" capacity and long-term proliferation potential of AML cells. There was considerably heterogeneity with regard to the effects of individual growth factors, but in general, IL-3, GM-CSF, and G-CSF promoted self-renewal of AML cells, and combinations tended to be more effective in supporting long-term survival of AML-CFU. There was evidence of gradual differentiation, but this was evident in control cells and did not appear to be accelerated by CSF treatment. These results of short-term and long-term cultures indicate that each of the CSFs tested can be used by AML cells to support proliferation. The lack of evidence that the CSFs enhance in vitro differentiation does not suggest they will be valuable as therapeutic differentiation agents.
为了进一步确定急性髓性白血病（AML）细胞体外增殖所需的生长因子，我们比较了重组白细胞介素-3（IL-3）、粒细胞-巨噬细胞集落刺激因子（GM-CSF）和粒细胞集落刺激因子（G-CSF）支持AML集落形成细胞（AML-CFU）生长的能力。IL-3、GM-CSF和G-CSF在短期集落培养中作为单一生长因子具有活性，并且在某些情况下联合使用时具有相加作用。我们还研究了这些集落刺激因子（CSF）对长期细胞悬浮培养中AML细胞增殖的影响。这些培养提供了对AML细胞“自我更新”能力和长期增殖潜力的估计。关于单个生长因子的作用存在相当大的异质性，但总体而言，IL-3、GM-CSF和G-CSF促进了AML细胞的自我更新，并且联合使用在支持AML-CFU的长期存活方面往往更有效。有逐渐分化的证据，但这在对照细胞中很明显，并且似乎没有因CSF处理而加速。短期和长期培养的这些结果表明，所测试的每种CSF都可以被AML细胞用于支持增殖。缺乏CSF增强体外分化的证据并不表明它们作为治疗性分化剂将是有价值的。
