人脐带华通氏胶源间充质干细胞的玻璃化冷冻保存
Vitrification of human umbilical cord Wharton's jelly-derived mesenchymal stem cells.
作者信息
Massood Ezzatabadipour, Maryam Kaviani, Parvin Salehinejad, Mojgan Mohammadi, Noureddin Nematollahi-Mahani Seyed
机构信息
Physiology Research Centre, Kerman University of Medical Sciences, Kerman, Iran.
Department of Anatomy, Medical School, Kerman University of Medical Sciences, Kerman, Iran.
出版信息
Cryo Letters. 2013 Sep-Oct;34(5):471-80.
The aim of this study was to evaluate whether vitrification is an effective cryopreservation method for storage of human umbilical cord mesenchymal cells (HUCMs). HUCMs were vitrified using a two step method. The viability of vitrified HUCMs (v-HUCM) and non-vitrified HUCMs (n-HUCM) was determined by Trypan Blue staining. The expression of several markers was evaluated using flow cytometry. The osteogenic, adipogenic and chondrogenic differentiation potential of v-HUCMs and n-HUCMs was determined. The post-warming viability of HUCMs was 95.54 +/- 2.30. The expression of surface antigens (strong positive for CD44, CD90 and CD105; negative for CD34 and CD45) was similar in the above mentioned groups. The v-HUCM cells retained ability to differentiate into osteoblasts, adipocytes and chondrocytes under appropriate culture conditions. The analysis of these results showed that vitrification is a reliable and effective method for cryopreservation of human umbilical cord mesenchymal cells.
本研究的目的是评估玻璃化是否是一种用于储存人脐带间充质细胞（HUCMs）的有效冷冻保存方法。HUCMs采用两步法进行玻璃化处理。通过台盼蓝染色测定玻璃化HUCMs（v-HUCM）和未玻璃化HUCMs（n-HUCM）的活力。使用流式细胞术评估几种标志物的表达。测定v-HUCMs和n-HUCMs的成骨、成脂和成软骨分化潜能。HUCMs复温后的活力为95.54±2.30。上述组中表面抗原的表达（CD44、CD90和CD105呈强阳性；CD34和CD45呈阴性）相似。v-HUCM细胞在适当的培养条件下保留了分化为成骨细胞、脂肪细胞和软骨细胞的能力。对这些结果的分析表明，玻璃化是一种可靠且有效的人脐带间充质细胞冷冻保存方法。
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