Ali Daoud, Ahmed Mukhtar, Alarifi Saud, Ali Huma
Department of Zoology, College of Science, King Saud University, Riyadh, 11451, Saudi Arabia.
Department of Chemistry, Maulana Azad National Institute of Technology, Bhopal, Madhya Pradesh, India.
Environ Toxicol. 2015 May-Jun;30(6):674-82. doi: 10.1002/tox.21945. Epub 2014 Jan 21.
Mammalian studies have raised concerns about the toxicity of carbon nanotubes, but there is very limited data on ecogenotoxicity to aquatic organisms. The aim of this study was to determine eco-geno toxic effects of single walled carbon nanotubes (SWCNTs) in fresh water snail, Lymnea luteola (L. luteola). A static test system was used to expose L. luteola to a freshwater control, 0.05, 0.15, 0.30, 0.46 mg/L SWCNTs for up to 4 days. SWCNTs changed a significant reduction in glutathione, glutathione-S-transferase, and glutathione peroxidase with in hepatopancreas of L. luteola. Lipid peroxidation (LPO) and catalase showed dose- and time-dependent and statistically significant increase in hepatopancreas during SWCNTs exposure compared with control. However, a significant (p < 0.01) induction in DNA damage was observed by the comet assay in hepatopancreas cells treated with SWCNTs. These results demonstrate that SWCNTs are ecogenotoxic to freshwater snail L. luteola. The oxidative stress and comet assay can successfully be used as sensitive tools of aquatic pollution biomonitoring.
哺乳动物研究引发了对碳纳米管毒性的担忧，但关于其对水生生物的生态遗传毒性的数据非常有限。本研究的目的是确定单壁碳纳米管（SWCNTs）对淡水螺黄斑沼螺（Lymnea luteola）的生态遗传毒性效应。采用静态试验系统，将黄斑沼螺暴露于淡水对照组、0.05、0.15、0.30、0.46 mg/L的SWCNTs中，持续4天。SWCNTs导致黄斑沼螺肝胰腺中的谷胱甘肽、谷胱甘肽-S-转移酶和谷胱甘肽过氧化物酶显著减少。与对照组相比，在SWCNTs暴露期间，肝胰腺中的脂质过氧化（LPO）和过氧化氢酶呈现出剂量和时间依赖性的统计学显著增加。然而，在用SWCNTs处理的肝胰腺细胞中，彗星试验观察到DNA损伤有显著（p < 0.01）诱导。这些结果表明，SWCNTs对淡水螺黄斑沼螺具有生态遗传毒性。氧化应激和彗星试验可成功用作水生污染生物监测的敏感工具。
