Cörekçi B, Irgın C, Halıcıoğlu K, Dursun S, Yavuz M Z
Department of Orthodontics, Faculty of Dentistry, Abant İzzet Baysal University, Bolu, Turkey
Department of Orthodontics, Faculty of Dentistry, Abant İzzet Baysal University, Bolu, Turkey.
Hum Exp Toxicol. 2014 Oct;33(10):1000-7. doi: 10.1177/0960327113520020. Epub 2014 Jan 21.
The aim of this study was to evaluate, the cytotoxicity of orthodontic composites in vitro as a function of degree of conversion (DC) and the light curing units (LCU) employed on mouse fibroblast (L929).
Cured samples of the composites Light bond (Reliance Orthodontic Products, Itasca, Illinois, USA), Ortho bracket paste (Bisco, Schaumburg, Illinois, USA), Opal bond MV (OPAL, South Jordan, Utah, USA), and Transbond XT (3M, Monrovia, California, USA) were prepared. Polymerization was performed with two LCUs: VALO Ortho (Ultradent, South Jordan, Utah, USA) is a third-generation LCU and Elipar S10 (3M, USA) is a second-generation LCU. Four samples were immersed in cell culture medium to obtain composite extracts. After incubation of L929 cell cultures with the extracts obtained, cytotoxicity was determined using the methyl tetrazolium test. Fourier transform infrared spectroscopy (FTIR) was used to evaluate DC for five samples. A multivariate analysis of variance (ANOVA), two-way ANOVA, and Tukey's honestly significant difference test were utilized for statistical analyses.
Cytotoxicity and DC of all tested composites (p < 0.001) and the interaction between composites and LCUs (p < 0.01) were significantly different. LCUs had no significant influence on the cytotoxicity and DC of composite materials (p > 0.05). The correlations between cell viability and DC were positive for three composites but statistically insignificant.
Composites and LCUs must be matched with one another to result in satisfactory maximal biocompatibility and DC. Opal Bond plasma light-emitting diode combination was a better choice for cell viability. Three composites showed a positive correlation between cytotoxicity and DC. Therefore high-intensity LCUs can be said to efficiently affect polymerization, and so, higher DC rates may achieve higher cell viability rates.
本研究旨在评估正畸复合材料在体外对小鼠成纤维细胞（L929）的细胞毒性，该毒性是转化率（DC）和所使用的光固化装置（LCU）的函数。
制备复合材料Light bond（美国伊利诺伊州伊塔斯卡的Reliance正畸产品公司）、Ortho bracket paste（美国伊利诺伊州绍姆堡的Bisco公司）、Opal bond MV（美国犹他州南乔丹的OPAL公司）和Transbond XT（美国加利福尼亚州蒙罗维亚的3M公司）的固化样品。使用两种LCU进行聚合：VALO Ortho（美国犹他州南乔丹的Ultradent公司）是第三代LCU，Elipar S10（美国3M公司）是第二代LCU。将四个样品浸入细胞培养基中以获得复合材料提取物。在用获得的提取物孵育L929细胞培养物后，使用甲基四氮唑试验测定细胞毒性。使用傅里叶变换红外光谱（FTIR）评估五个样品的DC。采用多因素方差分析（ANOVA）、双向ANOVA和Tukey真实显著差异检验进行统计分析。
所有测试复合材料的细胞毒性和DC（p < 0.001）以及复合材料与LCU之间的相互作用（p < 0.01）均有显著差异。LCU对复合材料的细胞毒性和DC没有显著影响（p > 0.05）。三种复合材料的细胞活力与DC之间的相关性为正，但无统计学意义。
复合材料和LCU必须相互匹配，以实现令人满意的最大生物相容性和DC。Opal Bond等离子体发光二极管组合对细胞活力是更好的选择。三种复合材料的细胞毒性与DC之间呈正相关。因此，可以说高强度LCU能有效影响聚合，所以，更高的DC率可能实现更高的细胞活力率。
