Xue Yuan, Chen Jianfei, Wang Yulong, Zhang Yanlong, Liu Dan, Hua Yuping
College of Wildlife Resources of Northeast Forest University, Harbin 150040, Heilongjiang, People's Republic of China.
State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, Heilongjiang, People's Republic of China.
J Zoo Wildl Med. 2013 Dec;44(4):951-6. doi: 10.1638/2013-0020R2.1.
The present study was undertaken to identify and characterize integrons and integrated resistance gene cassettes among multidrug resistant Escherichia coli isolates from a captive population of Amur tigers (Panthera tigris altaica) in China. In addition, the prevalence of antimicrobial resistance and class I integrons was assessed in E. coli strains (n = 61) isolated from a captive population of Amur tigers in Heilongjiang Amur Tiger Park, China. Among the isolates, 52.46% (32 of 61) were positive for intI1, but no isolates carried intI2 or intI3. Most isolates were susceptible to amoxicillin/clavulanic acid, aztreonam, and polymyxin B, while they also exhibited high incidence rates of resistance to ampicillin, doxycycline, chloramphenicol, tetracycline, and dihydrofolate reductase. Sequencing analysis revealed three gene cassettes, which encoded resistance to dihydrofolate reductase (dfrA15), dihydrofolate reductase (dfrA12), and adenyltransferase (aadA2). The gene cassette arrays dfrA15 (31%) and dfrA12-aadA2 (19%) were most prevalent among these isolates.
本研究旨在鉴定和表征来自中国圈养东北虎（Panthera tigris altaica）群体的多重耐药大肠杆菌分离株中的整合子及整合的耐药基因盒。此外，还评估了从中国黑龙江东北虎林园圈养东北虎群体中分离出的大肠杆菌菌株（n = 61）的耐药性和I类整合子的流行情况。在这些分离株中，52.46%（61株中的32株）intI1呈阳性，但没有分离株携带intI2或intI3。大多数分离株对阿莫西林/克拉维酸、氨曲南和多粘菌素B敏感，同时它们对氨苄西林、强力霉素、氯霉素、四环素和二氢叶酸还原酶也表现出较高的耐药率。测序分析揭示了三个基因盒，它们分别编码对二氢叶酸还原酶（dfrA15）、二氢叶酸还原酶（dfrA12）和腺苷酸转移酶（aadA2）的耐药性。基因盒阵列dfrA15（31%）和dfrA12-aadA2（19%）在这些分离株中最为普遍。
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