Oparina N Yu, Snezhkina A V, Sadritdinova A F, Veselovskii V A, Dmitriev A A, Senchenko V N, Mel'nikova N V, Speranskaya A S, Darii M V, Stepanov O A, Barkhatov I M, Kudryavtseva A V
Genetika. 2013 Jul;49(7):814-23. doi: 10.7868/s0016675813050111.
Glycolysis is a main catabolic pathway of glucose metabolism, accompanied by ATP synthesis. More than 30 enzymes are involved in glycolysis, and genes that encode them can be considered housekeeping genes due to the high conservatism and evolutionary antiquity of the process. We studied the expression of these genes in kidney papillary cancer and planocellular lung cancer via the bioinformatic analysis of transcriptome database and method of quantitative real time PCR. Quantitative analysis of mRNA level demonstrated that only a part ofgenes that encode glycolysis enzymes maintain relatively stable mRNA level, including the HK1, ADPGK, GPI, PGK1, and PKM2 genes in kidney papillary cancer and the ADPGK, ALDOA, GAPDH, PGK1, BPGM, ENO1, and PKM2 genes in planocellular lung cancer. The frequent increase in the mRNA expression of PFKP, ALDOA, and GAPDH genes in kidney cancer, as well as the GPI gene in lung cancer, were detected for the first time by real time PCR. For other genes, their differential expression was demonstrated; the cases of both a decrease and increase in the mRNA level were detected. Thus, several genes that can be used as control genes in transcriptome analysis by real time PCR in kidney and lung cancer, as well as a number of differentially expressed genes that can be potential oncomarkers, were identified.
糖酵解是葡萄糖代谢的主要分解代谢途径，伴随着ATP的合成。超过30种酶参与糖酵解过程，由于该过程具有高度保守性和进化古老性，编码这些酶的基因可被视为管家基因。我们通过对转录组数据库的生物信息学分析和定量实时PCR方法，研究了这些基因在肾乳头状癌和肺扁平细胞癌中的表达情况。mRNA水平的定量分析表明，只有一部分编码糖酵解酶的基因保持相对稳定的mRNA水平，包括肾乳头状癌中的HK1、ADPGK、GPI、PGK1和PKM2基因，以及肺扁平细胞癌中的ADPGK、ALDOA、GAPDH、PGK1、BPGM、ENO1和PKM2基因。通过实时PCR首次检测到肾癌中PFKP、ALDOA和GAPDH基因以及肺癌中GPI基因的mRNA表达频繁增加。对于其他基因，其差异表达也得到了证实；检测到mRNA水平既有下降也有上升的情况。因此，确定了一些可在肾癌和肺癌实时PCR转录组分析中用作对照基因的基因，以及一些可能作为潜在肿瘤标志物的差异表达基因。
