Morales-Polanco Fabian, Bates Christian, Lui Jennifer, Casson Joseph, Solari Clara A, Pizzinga Mariavittoria, Forte Gabriela, Griffin Claire, Garner Kirsten E L, Burt Harriet E, Dixon Hannah L, Hubbard Simon, Portela Paula, Ashe Mark P
School of Biological Sciences, Faculty of Biology, Medicine and Health, The University of Manchester, Manchester Academic Health Science Centre, Michael Smith Building, Oxford Road, Manchester M13 9PT, UK.
Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, IQUIBICEN-CONICET, Buenos Aires, Argentina.
iScience. 2021 Jan 19;24(2):102069. doi: 10.1016/j.isci.2021.102069. eCollection 2021 Feb 19.
Glycolysis is a fundamental metabolic pathway for glucose catabolism across biology, and glycolytic enzymes are among the most abundant proteins in cells. Their expression at such levels provides a particular challenge. Here we demonstrate that the glycolytic mRNAs are localized to granules in yeast and human cells. Detailed live cell and smFISH studies in yeast show that the mRNAs are actively translated in granules, and this translation appears critical for the localization. Furthermore, this arrangement is likely to facilitate the higher level organization and control of the glycolytic pathway. Indeed, the degree of fermentation required by cells is intrinsically connected to the extent of mRNA localization to granules. On this basis, we term these granules, core fermentation (CoFe) granules; they appear to represent translation factories, allowing high-level coordinated enzyme synthesis for a critical metabolic pathway.
糖酵解是生物界葡萄糖分解代谢的基本代谢途径，糖酵解酶是细胞中最丰富的蛋白质之一。它们在如此高的水平上表达带来了特殊的挑战。在这里，我们证明糖酵解mRNA定位于酵母和人类细胞中的颗粒。在酵母中进行的详细活细胞和单分子荧光原位杂交（smFISH）研究表明，mRNA在颗粒中被积极翻译，并且这种翻译对于定位似乎至关重要。此外，这种排列可能有助于糖酵解途径的更高级组织和控制。实际上，细胞所需的发酵程度与mRNA定位于颗粒的程度内在相关。在此基础上，我们将这些颗粒称为核心发酵（CoFe）颗粒；它们似乎代表了翻译工厂，允许为关键代谢途径进行高水平的协同酶合成。
