Bagan Jose, Sáez Guillermo T, Tormos M Carmen, Gavalda-Esteve Carmen, Bagan Leticia, Leopoldo-Rodado Manuel, Calvo Javier, Camps Carlos
Department of Oral Medicine, Valencia University, Valencia, Spain; Service of Stomatology and Maxillofacial Surgery, University General Hospital, Valencia, Spain.
J Oral Pathol Med. 2014 May;43(5):371-7. doi: 10.1111/jop.12151. Epub 2014 Jan 23.
To analyze whether oxidative stress (OS) changes are present in patients with bisphosphonate-related osteonecrosis of the jaw (BRONJ) versus controls.
Oxidative stress was analyzed in serum and unstimulated saliva of three groups: Group 1 consisted of 24 patients who had been treated with intravenous bisphosphonates (ivBPs) and developed BRONJ, group 2 consisted of 20 patients who had received ivBPs and did not develop BRONJ, and group 3 comprised 17 control subjects. Reduced glutathione (GSH), malondialdehyde (MDA), oxidized glutathione (GSSG), and 8-oxo-7,8-dihydro-2-deoxyguanosine (8-oxo-dG) levels, as well as the GSSG/GSH ratio, were measured.
Mean serum and saliva levels of MDA, GSSG, and 8-oxo-dG and the GSSG/GSH ratio were significantly higher in patients with BRONJ than in controls. We found no significant difference in OS according to BRONJ stage, sex, or location in the jaws. Logistic regression analysis revealed that the GSSG/GSH ratio was a significant factor predicting the development of BRONJ (P = 0.01).
Oxidative stress was detected in patients with BRONJ, and the GSSG/GSH ratio was the most significant OS variable found; it was a significant factor predicting the development of BRONJ.
分析双膦酸盐相关颌骨坏死（BRONJ）患者与对照组相比是否存在氧化应激（OS）变化。
对三组患者的血清和未刺激唾液中的氧化应激进行分析：第一组由24例接受静脉注射双膦酸盐（ivBPs）并发生BRONJ的患者组成，第二组由20例接受ivBPs但未发生BRONJ的患者组成，第三组由17名对照受试者组成。测量还原型谷胱甘肽（GSH）、丙二醛（MDA）、氧化型谷胱甘肽（GSSG）和8-氧代-7,8-二氢-2'-脱氧鸟苷（8-氧代-dG）水平以及GSSG/GSH比值。
BRONJ患者的血清和唾液中MDA、GSSG和8-氧代-dG的平均水平以及GSSG/GSH比值显著高于对照组。根据BRONJ分期、性别或颌骨位置，我们发现OS无显著差异。逻辑回归分析显示，GSSG/GSH比值是预测BRONJ发生的重要因素（P = 0.01）。
在BRONJ患者中检测到氧化应激，GSSG/GSH比值是发现的最显著的OS变量；它是预测BRONJ发生的重要因素。
