Int J Oral Maxillofac Implants. 2014 Jan-Feb;29(1):221-31. doi: 10.11607/jomi.3186.
To evaluate the effects of caffeine and/or estrogen deficiency on trabecular bone area (TBA) and bone healing in rats.
Rats were divided into groups (n=15/group) as follows: control, caffeine, ovariectomy (OVX), and caffeine/OVX. Critical-sized defects were created in the tibiae (57 days after beginning caffeine administration and 43 days after OVX). The intact femurs were evaluated for TBA and the number of positive cells for tartrate-resistant acid phosphatase (TRAP), receptor activator of nuclear factor-κB ligand (RANKL), and osteoprotegerin (OPG). In the defects, bone healing, the number of TRAP+ and RANKL/OPG+ cells, and gene expression of bone morphogenetic protein (BMP)-2, BMP-7, osteopontin, and CBP/p300-interacting-transactivator-with-ED-rich-tail-2 (CITED-2) were evaluated.
Bone healing was poorer in defects of the caffeine group than in those of the control group. The femurs of the OVX and OVX/caffeine groups presented lower TBAs and higher RANKL/OPG+ cell ratios. The number of TRAP+ cells was higher in femurs of the caffeine group and in defects of the OVX group. The caffeine/OVX group presented the highest RANKL/OPG+ cell ratio in femurs and defects. The OVX group presented the highest expression of BMP-2, BMP-7, and CITED-2.
Caffeine affected bone healing, while estrogen deficiency mainly affected TBA, but no significant deleterious synergic effects of both conditions were observed.
评估咖啡因和/或雌激素缺乏对大鼠小梁骨面积（TBA）和骨愈合的影响。
将大鼠分为以下几组（每组 15 只）：对照组、咖啡因组、卵巢切除术（OVX）组和咖啡因/OVX 组。在开始给予咖啡因 57 天后和 OVX 后 43 天，在胫骨中造成临界尺寸的缺损。评估完整股骨的 TBA 和抗酒石酸酸性磷酸酶（TRAP）阳性细胞数、核因子-κB 配体（RANKL）和骨保护素（OPG）。在缺陷中，评估骨愈合、TRAP+和 RANKL/OPG+细胞的数量以及骨形态发生蛋白（BMP）-2、BMP-7、骨桥蛋白和 CBP/p300 相互作用转录激活因子与 ED 富含尾巴 2（CITED-2）的基因表达。
与对照组相比，咖啡因组缺陷中的骨愈合较差。OVX 和 OVX/咖啡因组的股骨 TBA 较低，RANKL/OPG+细胞比值较高。咖啡因组股骨和 OVX 组缺陷中的 TRAP+细胞数量较高。在股骨和缺陷中，咖啡因/OVX 组的 RANKL/OPG+细胞比值最高。OVX 组表现出最高的 BMP-2、BMP-7 和 CITED-2 表达。
咖啡因影响骨愈合，而雌激素缺乏主要影响 TBA，但两种情况没有明显的协同有害作用。
