Casarin R C, Casati M Z, Pimentel S P, Cirano F R, Algayer M, Pires P R, Ghiraldini B, Duarte P M, Ribeiro F V
Dental Research Division, School of Dentistry, Paulista University, São Paulo, SP, Brazil.
Department of Periodontology, Dental Research Division, Guarulhos University, Guarulhos, SP, Brazil.
Int J Oral Maxillofac Surg. 2014 Jul;43(7):900-6. doi: 10.1016/j.ijom.2014.01.009. Epub 2014 Feb 13.
This study investigated the effect of resveratrol on bone healing and its influence on the gene expression of osteogenic markers. Two calvarial defects were created and one screw-shaped titanium implant was inserted in the tibia of rats that were assigned to daily administration of placebo (control group, n=15) or 10mg/kg of resveratrol (RESV group, n=15) for 30 days. The animals were then sacrificed. One of the calvarial defects was processed for histomorphometric analysis and the tissue relative to the other was collected for mRNA quantification of bone morphogenetic protein (BMP)-2, BMP-7, osteopontin (OPN), bone sialoprotein (BSP), osteoprotegrin (OPG), and receptor activator of NF-κB ligand (RANKL). Implants were removed by applying a counter-torque force. Histomorphometric analysis revealed higher remaining defect in the calvarial defects of the control group than the RESV group (P=0.026). Resveratrol increased the counter-torque values of implant removal when compared to control therapy (P=0.031). Gene expression analysis showed a higher expression of BMP-2 (P=0.011), BMP-7 (P=0.049), and OPN (P=0.002) genes in the RESV group than in the control group. In conclusion, resveratrol improved the repair of critical-sized bone defects and the biomechanical retention of implants. Indeed, this natural agent may up-regulate the gene expression of important osteogenic markers.
本研究调查了白藜芦醇对骨愈合的影响及其对成骨标志物基因表达的作用。在大鼠颅骨制造两个缺损，并在其胫骨植入一枚螺旋形钛植入物，将大鼠分为两组，分别每日给予安慰剂（对照组，n = 15）或10mg/kg白藜芦醇（RESV组，n = 15），持续30天。然后处死动物。其中一个颅骨缺损用于组织形态计量学分析，另一个相关组织用于定量检测骨形态发生蛋白（BMP）-2、BMP-7、骨桥蛋白（OPN）、骨唾液蛋白（BSP）、骨保护素（OPG）和核因子κB受体活化因子配体（RANKL）的mRNA。通过施加反向扭矩力取出植入物。组织形态计量学分析显示，对照组颅骨缺损的剩余缺损高于RESV组（P = 0.026）。与对照治疗相比，白藜芦醇增加了植入物取出的反向扭矩值（P = 0.031）。基因表达分析表明，RESV组中BMP-2（P = 0.011）、BMP-7（P = 0.049）和OPN（P = 0.002）基因的表达高于对照组。总之，白藜芦醇改善了临界尺寸骨缺损的修复及植入物的生物力学固定。实际上，这种天然物质可能上调重要成骨标志物的基因表达。
