通过银染法在聚丙烯酰胺凝胶上检测几皮克DNA。
Detection of a few picograms of DNA on polyacrylamide gels by silver staining.
作者信息
Lomholt B, Frederiksen S
机构信息
Institute of Medical Genetics, Panum Institute, University of Copenhagen, Denmark.
出版信息
Anal Biochem. 1987 Jul;164(1):146-9. doi: 10.1016/0003-2697(87)90378-2.
DNA fragments separated on polyacrylamide gels are silver stained in ethanolamine solution. The staining procedure can be completed in 3 1/2 h. Illumination of the gels on a black background increases the sensitivity of detection compared with the usual transillumination. The limit level of detection is 3-5 pg per band with a cross-sectional area of 5 mm2. Five to fifty picograms of DNA may be detected quantitatively by scanning the gels. The method will detect 0.1 to 1 ng per band of low-molecular-weight RNA components.
在聚丙烯酰胺凝胶上分离的DNA片段在乙醇胺溶液中进行银染。染色过程可在3.5小时内完成。与常规透射照明相比，在黑色背景下对凝胶进行照明可提高检测灵敏度。对于横截面积为5平方毫米的条带，检测限为每条带3-5皮克。通过扫描凝胶可对5至50皮克的DNA进行定量检测。该方法可检测每条带0.1至1纳克的低分子量RNA成分。
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