Halili Albana Ndreu, Hasirci Nesrin, Hasirci Vasif
Department of Biotechnology, METU, 06800, Ankara, Turkey.
J Mater Sci Mater Med. 2014 Apr;25(4):1195-209. doi: 10.1007/s10856-014-5145-0. Epub 2014 Jan 23.
Various methods have been tried to treat the main meniscus problem, meniscal tears, for which we believe tissue engineering could be a viable solution. In this study, a three dimensional, collagen-based meniscus substitute was prepared by tissue engineering using human fibrochondrocytes and a collagen based-scaffold. This construct was made with 3 different collagen-based foams interspaced with two electrospun nano/microfibrous mats. The top layer was made of collagen type I-chondroitin sulfate-hyaluronic acid (Coll-CS-HA), and the middle and the bottom layers were made of only collagen type I with different porosities and thus with different mechanical properties. The mats of aligned fibers were a blend of collagen type I and poly(L-lactic acid-co-glycolic acid) (PLGA). After seeding with human fibrochondrocytes, cell attachment, proliferation, and production of extracellular matrix and glucoseaminoglycan were studied. Cell seeding had a positive effect on the compressive properties of foams and the 3D construct. The 3D construct with all its 5 layers had better mechanical properties than the individual foams.
人们已经尝试了各种方法来治疗半月板的主要问题——半月板撕裂，我们认为组织工程学可能是一个可行的解决方案。在本研究中，使用人纤维软骨细胞和基于胶原蛋白的支架，通过组织工程制备了一种三维的、基于胶原蛋白的半月板替代物。该构建体由3种不同的基于胶原蛋白的泡沫组成，中间间隔有两层电纺纳米/微纤维垫。顶层由I型胶原蛋白-硫酸软骨素-透明质酸（Coll-CS-HA）制成，中间层和底层仅由具有不同孔隙率和不同机械性能的I型胶原蛋白制成。排列的纤维垫是I型胶原蛋白和聚（L-乳酸-共-乙醇酸）（PLGA）的混合物。接种人纤维软骨细胞后，研究了细胞附着、增殖以及细胞外基质和葡萄糖胺聚糖的产生。细胞接种对泡沫和三维构建体的压缩性能有积极影响。具有所有五层的三维构建体比单个泡沫具有更好的机械性能。
