利用新型柔性环状聚酰胺对 c-myb 癌基因启动子中 G-四链体的选择性识别进行 ESI 质谱探测。
ESI mass spectrometric exploration of selective recognition of G-quadruplex in c-myb oncogene promoter using a novel flexible cyclic polyamide.
机构信息
Beijing National Laboratory for Molecular Sciences, Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education, Department of Chemical Biology, College of Chemistry and Molecular Engineering, Peking University, Beijing, 100871, China.
出版信息
J Am Soc Mass Spectrom. 2014 Apr;25(4):684-91. doi: 10.1007/s13361-013-0802-y. Epub 2014 Jan 23.
In this research, electrospray ionization mass spectrometry (ESI-MS) was used to probe the binding selectivity of a flexible cyclic polyamide (cβ) to G-quadruplexes from the long G-rich sequences in the c-myb oncogene promoter. The results show that three G-rich sequences, including d[(GGA)3GGTCAC(GGA)4], d[(GGA)4GAA(GGA)4], and d[(GGA)3GGTCAC(GGA)4GAA(GGA)4] species in the c-myb promoter can form parallel G-quadruplexes, and cβ selectively binds towards these G-quadruplexes over both several other G-quadruplexes and the duplex DNA. These properties of cβ have profound implications on future studies of the regulation of c-myb oncogene expression.
在这项研究中，电喷雾电离质谱（ESI-MS）被用于探测柔性环状聚酰胺（cβ）与 c-myb 癌基因启动子中富含 G 的长序列形成的 G-四链体的结合选择性。结果表明，c-myb 启动子中的三个富含 G 的序列，包括 d[(GGA)3GGTCAC(GGA)4]、d[(GGA)4GAA(GGA)4]和 d[(GGA)3GGTCAC(GGA)4GAA(GGA)4]，可以形成平行的 G-四链体，cβ选择性地与这些 G-四链体结合，而不是与其他几个 G-四链体和双链 DNA 结合。cβ的这些特性对未来研究 c-myb 癌基因表达的调控具有深远的意义。
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