Shipley G D, Pittelkow M R
Department of Cell Biology and Anatomy, School of Medicine, Oregon Health Sciences University, Portland 97201.
Arch Dermatol. 1987 Nov;123(11):1541a-1544a.
Normal human proliferative keratinocytes can be serially cultured in serum-free medium. The medium and culture conditions that have been developed support the growth of these cells in the absence of a feeder layer and in the absence of contaminating fibroblasts. Using this system, the nutritional and hormonal factors that affect the growth and differentiation of these cells have been studied in the absence of undefined supplements. The data suggest that the control of proliferation and differentiation in keratinocytes may take place by unique mechanisms. The defined serum-free conditions that have been developed provide an excellent system for the study of nutritional disorders that affect human epidermal cells. In addition, the culture system can be used to propagate large numbers of cells for use in autografts for cases of severe burns.
正常人类增殖性角质形成细胞可在无血清培养基中连续培养。已开发的培养基和培养条件支持这些细胞在没有饲养层且没有成纤维细胞污染的情况下生长。利用该系统，在没有未定义补充剂的情况下，研究了影响这些细胞生长和分化的营养和激素因素。数据表明，角质形成细胞增殖和分化的控制可能通过独特的机制进行。已开发的确定无血清条件为研究影响人类表皮细胞的营养紊乱提供了一个极好的系统。此外，该培养系统可用于大量繁殖细胞，用于严重烧伤病例的自体移植。
