Australian Equine Laminitis Research Unit, School of Veterinary Science, The University of Queensland, Gatton, Queensland, Australia.
Department of Clinical Studies, School of Veterinary Medicine, New Bolton Center, University of Pennsylvania, Kennett Square, Pennsylvania, USA.
J Vet Intern Med. 2021 May;35(3):1589-1596. doi: 10.1111/jvim.16095. Epub 2021 Mar 11.
Endocrinopathic laminitis develops in association with insulin dysregulation, but the role of insulin in the pathogenesis remains unclear. Hyperinsulinemia can cause hypoaminoacidemia, which is associated with integumentary lesions in other species and therefore warrants investigation as a potential mechanism in laminitis.
Evaluate plasma amino acid concentrations in the euglycemic-hyperinsulinemic clamp (EHC) and prolonged glucose infusion (PGI) laminitis models.
Sixteen Standardbred horses.
Prospective experimental study. Plasma amino acid concentrations were measured in samples collected every 6 hours from horses that underwent a 48-hour EHC (n = 8) or 66-hour PGI (n = 8) after a 24- or 6-hour baseline period in EHC and PGI groups, respectively.
Fifteen of the 20 measured amino acid concentrations decreased over time in both EHC and PGI horses (P < 0.001). The median percentage change from baseline for these amino acids was: histidine (EHC: 41.5%; PGI: 43.9%), glutamine (EHC: 51.8%; PGI: 35.3%), arginine (EHC: 51.4%; PGI: 41%), glutamic acid (EHC: 52.4%; PGI: 31.7%), threonine (EHC: 62.8%; PGI: 25.2%), alanine (EHC: 48.9%; PGI: 19.5%), proline (EHC: 56.2%; PGI: 30.3%), cystine (EHC: 34.9%; PGI: 31.2%), lysine (EHC: 46.4%; PGI: 27.8%), tyrosine (EHC: 27.5%; PGI: 16.9%), methionine (EHC: 69.3%; PGI: 50.8%), valine (EHC: 50.8%; PGI: 34.4%), isoleucine (EHC: 60.8%; PGI: 38.7%), leucine (EHC: 48.2%; PGI: 36.6%), and phenylalanine (EHC: 16.6%; PGI: 12.1%).
Hypoaminoacidemia develops in EHC and PGI laminitis models. The role of hypoaminoacidemia in the development of hyperinsulinemia-associated laminitis warrants further investigation.
内分泌性蹄叶炎与胰岛素失调有关，但胰岛素在发病机制中的作用尚不清楚。高胰岛素血症可导致低氨基酸血症，这与其他物种的皮肤损伤有关，因此有必要将其作为蹄叶炎的潜在机制进行研究。
评估正常血糖高胰岛素钳夹（EHC）和延长葡萄糖输注（PGI）蹄叶炎模型中的血浆氨基酸浓度。
16 匹标准马。
前瞻性实验研究。在 EHC 组中，24 小时基线期后进行 48 小时 EHC，PGI 组中进行 66 小时 PGI，分别于 EHC 和 PGI 组每 6 小时采集样本，测量血浆氨基酸浓度。
在 EHC 和 PGI 马中，20 种测量氨基酸浓度中的 15 种随时间推移而降低（P < 0.001）。这些氨基酸的中位数与基线相比的变化百分比为：组氨酸（EHC：41.5%；PGI：43.9%）、谷氨酰胺（EHC：51.8%；PGI：35.3%）、精氨酸（EHC：51.4%；PGI：41%）、谷氨酸（EHC：52.4%；PGI：31.7%）、苏氨酸（EHC：62.8%；PGI：25.2%）、丙氨酸（EHC：48.9%；PGI：19.5%）、脯氨酸（EHC：56.2%；PGI：30.3%）、半胱氨酸（EHC：34.9%；PGI：31.2%）、赖氨酸（EHC：46.4%；PGI：27.8%）、酪氨酸（EHC：27.5%；PGI：16.9%）、蛋氨酸（EHC：69.3%；PGI：50.8%）、缬氨酸（EHC：50.8%；PGI：34.4%）、异亮氨酸（EHC：60.8%；PGI：38.7%）、亮氨酸（EHC：48.2%；PGI：36.6%）和苯丙氨酸（EHC：16.6%；PGI：12.1%）。
在 EHC 和 PGI 蹄叶炎模型中出现低氨基酸血症。低氨基酸血症在高胰岛素血症相关蹄叶炎的发生中的作用值得进一步研究。
