一种用于快速诊断传染性法氏囊病的免疫过氧化物酶单克隆抗体染色剂。
An immunoperoxidase monoclonal antibody stain for rapid diagnosis of infectious bursal disease.
作者信息
Cho B R, Snyder D B, Lana D P, Marquardt W W
机构信息
Department of Veterinary Microbiology-Pathology, Washington State University, Pullman 99164.
出版信息
Avian Dis. 1987 Jul-Sep;31(3):538-45.
Cell smears of chicken-embryo-fibroblast (CEF) cultures and bursa of Fabricius from chickens experimentally infected with six different strains of infectious bursal disease virus (IBDV) were examined for the presence of IBDV by the avidin-biotin-peroxidase complex method of immunoperoxidase (IP) staining using a monoclonal antibody specific for IBDV designated BK70. IBDV of different strains and serotypes were readily detected by the IP method in cell smears prepared from infected CEF cultures and from bursas. Bursal cells were positive for IP stain in most of the infected bursas (87.5%), despite their mild IBD lesions. Positive IP staining of bursal smears was well correlated with the recovery of IBDV from the bursas and with IBD lesions in the bursas. IP stain with a monoclonal antibody (BK70) appeared potentially useful for rapid and definitive diagnosis of IBD.
采用抗传染性法氏囊病病毒（IBDV）单克隆抗体BK70，运用免疫过氧化物酶（IP）染色的抗生物素蛋白-生物素-过氧化物酶复合物法，对感染6种不同毒株IBDV的鸡胚成纤维细胞（CEF）培养物涂片以及鸡的法氏囊进行检查，以确定是否存在IBDV。通过IP法，在感染的CEF培养物和法氏囊制备的细胞涂片中，能够轻易检测到不同毒株和血清型的IBDV。尽管大多数感染法氏囊存在轻度IBD病变，但其中87.5%的法氏囊细胞IP染色呈阳性。法氏囊涂片的阳性IP染色与从法氏囊中分离出IBDV以及法氏囊中的IBD病变密切相关。用单克隆抗体（BK70）进行IP染色，似乎对IBD的快速确诊具有潜在作用。
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