In situ patch-clamp recording of calcium-activated potassium channels from an identified leech neuron.

The paired anterior lateral giant cells of the leech Haementeria have only two active voltage gated ionic currents. We took advantage of this simple complement of ionic currents to investigate the single channel properties of this cells' calcium-activated K+ current (I(K,Ca) in situ. Cell-attached patch recordings showed large, bursting events with a conductance of approximately 90-100 pS which had extrapolated reversal potential consistent with K+ events. The channel open time distribution was well described by a single exponential process while the channel closed times were bi-exponentially distributed. The results show that the single channel properties of I(K,Ca) in annelids closely resemble those of similar currents described in vertebrates.