Strassman A, Mason P, Eckenstein F, Baughman R W, Maciewicz R
Pain Physiology Laboratory, Massachusetts General Hospital, Boston 02114.
Brain Res. 1987 Oct 13;423(1-2):293-304. doi: 10.1016/0006-8993(87)90852-3.
The distribution of cholinergic neurons in the region of the cat Edinger-Westphal nucleus (EW) was determined by immunocytochemical localization of the acetylcholine-synthesizing enzyme choline acetyltransferase (ChAT). Neurons containing ChAT-like immunoreactivity (ChAT-LI) were densely distributed within EW, the anteromedian nucleus (AM), and the oculomotor nucleus (III), and were also present in immediately adjacent regions of the periaqueductal gray and ventral tegmental region. The majority of labelled neurons in EW and AM showed a markedly lower intensity of ChAT-LI than the labelled neurons in III and adjacent regions. To determine the relationship of cells with ChAT-LI to the distribution of ciliary ganglion afferent neurons, a double labelling immunocytochemistry/retrograde transport technique was also used. These experiments showed that many of the cells located outside of III that stained intensely for ChAT-LI project to ciliary ganglion. Very few ciliary ganglion afferent neurons were found in EW or AM itself; instead, the distribution of lightly labelled ChAT-LI-positive neurons in EW and AM more closely matched the known distribution of peptide-containing cells that have descending, central projections.
通过对乙酰胆碱合成酶胆碱乙酰转移酶（ChAT）进行免疫细胞化学定位，确定了猫动眼神经副核（EW）区域胆碱能神经元的分布。含有ChAT样免疫反应性（ChAT-LI）的神经元密集分布于EW、前正中核（AM）和动眼神经核（III）内，也存在于导水管周围灰质和腹侧被盖区的紧邻区域。EW和AM中大多数标记神经元的ChAT-LI强度明显低于III及其相邻区域中的标记神经元。为了确定具有ChAT-LI的细胞与睫状神经节传入神经元分布的关系，还采用了双重标记免疫细胞化学/逆行运输技术。这些实验表明，许多位于III之外且ChAT-LI染色强烈的细胞投射至睫状神经节。在EW或AM本身中发现的睫状神经节传入神经元极少；相反，EW和AM中轻度标记的ChAT-LI阳性神经元的分布与具有下行性中央投射的含肽细胞的已知分布更为匹配。
