Ueyama H, Nakayasu H, Ueda K
Department of Medical Biochemistry, Shiga University of Medical Science, Ohtsu, Japan.
Cell Biol Int Rep. 1987 Sep;11(9):671-7. doi: 10.1016/0309-1651(87)90102-0.
The role of nuclear actin filaments in the RNA transport was investigated. Mouse lymphoma cells, L5178Y, were labeled for 20 min with 3H-uridine, and the isolated nuclei were incubated in a medium consisting of 0.25 M sucrose, 10 mM Tris-HCl (pH 7.5), 2 mM CaCl2, 1 mM ATP and 1mM PMSF. Release of the rapidly labeled RNA from the nuclei was temperature-dependent and was stimulated by ATP. Phalloidin, an inhibitor of actin filament depolymerization, had no effect on the system at 10 or 100 micrograms/ml. Therefore, actin filament depolymerization may not be involved in the transport of RNA.
