小鼠T细胞和B细胞对甲状腺球蛋白自身抗原表位的识别。
Recognition of thyroglobulin autoantigenic epitopes by murine T and B cells.
作者信息
Champion B R, Page K, Rayner D C, Quartey-Papafio R, Byfield P G, Henderson G
机构信息
Department of Immunology, Middlesex Hospital Medical School, U.K.
出版信息
Immunology. 1987 Oct;62(2):255-63.
We have used a large panel of thyroglobulins (Tg) prepared from a wide range of mammalian species to study the Tg autoantigenic epitopes recognized by populations of monoclonal and polyclonal murine T and B cells. This approach showed the existence of at least six different epitopes; three recognized by T cells (in association with I-Ak on antigen-presenting cells) and three by B cells (monoclonal antibodies). The majority of serum and monoclonal autoantibodies were found to be highly specific for mouse Tg, with some cross-reactive binding to rat Tg. In contrast, T-cell lines/clones and hybridomas recognized cross-reactive epitopes on Tg that were highly conserved throughout most of the mammalian orders. Moreover, two hybrid clones, which showed similar patterns of cross-reactivity, differed in their responsiveness to tryptic digests of human Tg. Thus, autoreactive T and B cells recognize distinct areas of the Tg molecule.
我们使用了从多种哺乳动物物种制备的一大组甲状腺球蛋白（Tg），来研究单克隆和多克隆鼠T细胞及B细胞群体所识别的Tg自身抗原表位。该方法显示至少存在六种不同的表位；三种由T细胞识别（与抗原呈递细胞上的I-Ak相关联），三种由B细胞识别（单克隆抗体）。发现大多数血清和单克隆自身抗体对小鼠Tg具有高度特异性，对大鼠Tg有一些交叉反应性结合。相比之下，T细胞系/克隆和杂交瘤识别Tg上的交叉反应性表位，这些表位在大多数哺乳动物目中高度保守。此外，两个显示出相似交叉反应模式的杂交克隆，对人Tg的胰蛋白酶消化产物的反应性不同。因此，自身反应性T细胞和B细胞识别Tg分子的不同区域。
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