Parish N M, Rayner D, Cooke A, Roitt I M
Department of Immunology, Middlesex Hospital Medical School, London, U.K.
Immunology. 1988 Feb;63(2):199-203.
When mice are pretreated with soluble mouse thyroglobulin (MTg), subsequent induction of autoantibodies in experimental allergic thyroiditis (EAT) is suppressed. This suppression can be reproducibly transferred to low-level irradiated syngeneic recipients and is specific for MTg. Injection of normal cells does not reverse this tolerance, also indicative of an active suppression. Neither can the induced unresponsiveness be overcome by immunization with cross-reactive xenogeneic Tg; although antibodies are formed which will bind to MTg, these are not to epitopes to which antibodies are normally formed on immunization or towards which tolerance is induced. This implies that tolerance might be induced at least at the B-cell level, a view supported by the inability of DNP to provide a new carrier to break tolerance when conjugated to MTg. The poorer response to DNP in these animals also suggests anergy of the MTg-specific T helpers.
当用可溶性小鼠甲状腺球蛋白（MTg）对小鼠进行预处理时，实验性过敏性甲状腺炎（EAT）中自身抗体的后续诱导会受到抑制。这种抑制作用可重复转移至低剂量照射的同基因受体，且对MTg具有特异性。注射正常细胞并不能逆转这种耐受性，这也表明存在主动抑制。用交叉反应性异种甲状腺球蛋白（Tg）免疫也无法克服诱导的无反应性；尽管会形成能与MTg结合的抗体，但这些抗体针对的不是免疫时通常形成抗体的表位，也不是诱导耐受性时针对的表位。这意味着耐受性可能至少在B细胞水平被诱导，当DNP与MTg偶联时无法提供新的载体来打破耐受性，这一观点得到了支持。这些动物对DNP的反应较差也表明MTg特异性T辅助细胞存在无反应性。
