Wiaderkiewicz Jan, Głowacka Marta, Grabowska Marta, Jarosław-Jerzy Barski
Center for Experimental Medicine, Medical University of Silesia, Katowice, Poland,
Acta Neurobiol Exp (Wars). 2013;73(4):557-63. doi: 10.55782/ane-2013-1959.
Mice, similarly to some other rodent species, communicate with specialized sounds in the ultrasonic range called ultrasonic vocalizations (USV). Evaluation of this behavioral activity enables estimation of the social interactions in animal models of autistic spectrum disorders (ASD). Because transgenic mouse models are generated, in most cases, on the mixed 129SV/C57BL6 genetic background, we were interested if parameters that characterize USV differ between these two mouse strains. In addition, we wanted to compare these strains with the BALB/c line. In order to analyze USV, we applied the standard isolation test to newborn animals and compared standard parameters. Obtained results indicate clear differences between the 129SV and C57BL6 strains in respect to all analyzed USV parameters. Both strains behave also differently when compared with the BALB/c strain. For this reason in experiments utilizing transgenic animals, contribution of various genetic backgrounds has to be carefully considered.
与其他一些啮齿动物物种类似，小鼠通过超声波范围内的特殊声音进行交流，这种声音被称为超声波发声（USV）。对这种行为活动的评估能够估计自闭症谱系障碍（ASD）动物模型中的社交互动情况。由于转基因小鼠模型大多是在混合的129SV/C57BL6遗传背景下产生的，我们想知道表征USV的参数在这两种小鼠品系之间是否存在差异。此外，我们还想将这些品系与BALB/c品系进行比较。为了分析USV，我们对新生动物应用了标准隔离试验并比较了标准参数。获得的结果表明，在所有分析的USV参数方面，129SV和C57BL6品系之间存在明显差异。与BALB/c品系相比，这两个品系的行为也有所不同。因此，在利用转基因动物进行的实验中，必须仔细考虑各种遗传背景的影响。
