光照和黑暗对荚膜红假单胞菌 E1F1 硝酸盐同化的影响。
Effect of light and darkness on nitrate assimilation by Rhodopseudomonas capsulata E1F1.
机构信息
Departamento de Bioquímica, Facultad de Biología y C.S.I.C., Sevilla, Spain.
出版信息
Photosynth Res. 1982 Dec;3(4):313-9. doi: 10.1007/BF00034112.
The photosynthetic nonsulfur purple bacterium Rhodopseudomonas capsulata strain E1F1 assimilated nitrate or nitrite only in illuminated cultures under anaerobic conditions. The bacterial cells grew aerobically in the dark only when ammonia or other forms of reduced nitrogen were present in the medium. However, nitrate reductase was detected either in light-anaerobic or in dark-aerobic conditions upon addition of nitrate to the media. Changes from light-anaerobic to dark-aerobic conditions and vice versa markedly influenced growth, nitrate uptake and the nitrate reductase levels. Growth on nitrate in the light and nitrate reductase activity were dependent on the presence of molybdenum in the medium whereas the addition of tungstate inhibited both growth and enzyme activity.
光合非硫紫色细菌荚膜红假单胞菌 E1F1 菌株只能在厌氧光照条件下同化硝酸盐或亚硝酸盐。细菌细胞在黑暗中仅在有氧条件下生长，当培养基中存在氨或其他形式的还原氮时。然而，在向培养基中添加硝酸盐后，无论是在光厌氧条件下还是在暗需氧条件下，都检测到了硝酸盐还原酶。从光厌氧到暗需氧条件的变化和反之亦然，明显影响了生长、硝酸盐吸收和硝酸盐还原酶水平。在光照下利用硝酸盐生长和硝酸盐还原酶活性取决于培养基中钼的存在，而钨酸盐的添加则抑制了生长和酶活性。
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