McEwan A G, Greenfield A J, Wetzstein H G, Jackson J B, Ferguson S J
J Bacteriol. 1985 Nov;164(2):823-30. doi: 10.1128/jb.164.2.823-830.1985.
After growth in the absence of nitrogenous oxides under anaerobic phototrophic conditions, several strains of Rhodopseudomonas capsulata were shown to possess a nitrous oxide reductase activity. The enzyme responsible for this activity had a periplasmic location and resembled a nitrous oxide reductase purified from Pseudomonas perfectomarinus. Electron flow to nitrous oxide reductase was coupled to generation of a membrane potential and inhibited by rotenone but not antimycin. It is suggested that electron flow to nitrous oxide reductase branches at the level of ubiquinone from the previously characterized electron transfer components of R. capsulata. This pathway of electron transport could include cytochrome c', a component hitherto without a recognized function. R. capsulata grew under dark anaerobic conditions in the presence of malate as carbon source and nitrous oxide as electron acceptor. This confirms that nitrous oxide respiration is linked to ATP synthesis. Phototrophically and anaerobically grown cultures of nondenitrifying strains of Rhodopseudomonas sphaeroides, Rhodopseudomonas palustris, and Rhodospirillum rubrum also possessed nitrous oxide reductase activity.
在厌氧光合条件下于无氮氧化物环境中生长后，几株荚膜红假单胞菌被证明具有一氧化二氮还原酶活性。负责该活性的酶定位于周质，且类似于从完美海假单胞菌中纯化得到的一氧化二氮还原酶。流向一氧化二氮还原酶的电子流与膜电位的产生相偶联，并受鱼藤酮抑制，但不受抗霉素抑制。有人提出，流向一氧化二氮还原酶的电子流在泛醌水平上从荚膜红假单胞菌先前已鉴定的电子传递组分处分支。这条电子传递途径可能包括细胞色素c'，这是一种迄今尚无公认功能的组分。荚膜红假单胞菌在黑暗厌氧条件下，以苹果酸作为碳源、一氧化二氮作为电子受体生长。这证实了一氧化二氮呼吸与ATP合成相关。球形红假单胞菌、沼泽红假单胞菌和深红红螺菌的非反硝化菌株在光合和厌氧条件下生长的培养物也具有一氧化二氮还原酶活性。
