光敏色素的红光和远红光吸收形式对 [(14)C]N-乙基马来酰亚胺的差异反应性。

Differential reactivity of the red-and far-red-absorbing forms of phytochrome to [(14)C] N-ethyl maleimide.

机构信息

The Biological Laboratories, Harvard University, 02138, Cambridge, Massachusetts, USA.

出版信息

Planta. 1974 Dec;117(4):367-72. doi: 10.1007/BF00388032.

Abstract

The red-absorbing form (P r ) and the far-red absorbing form (P fr ) of undergraded, high-molecular-weight phytochrome from rye (Secale cereale L.) seedlings were examined for their reactivity toward N-ethyl-[(14)C]maleimide ([(14)C]-NEM). After pre-treatment of P r with cold NEM and extensive dialysis, photoconversion to P fr and treatment with [(14)C]NEM resulted in an approximately 70% increase in incorporation of radioactivity over the dark control. These results are discussed in relation to the view that phytochrome undergoes a protein conformational change upon phototransformation.

摘要

用 N-乙基-[(14)C]马来酰亚胺（[(14)C]-NEM）研究了从小麦（Secale cereale L.）幼苗中提取的、未经分级的高分子量光敏色素的红光吸收型（P r ）和远红光吸收型（P fr ）对其的反应性。P r 用冷 NEM 预处理并进行充分透析后，光转化为 P fr 并用 [(14)C]NEM 处理，其放射性掺入量比暗对照增加了约 70%。这些结果与光敏色素在光转化时发生蛋白质构象变化的观点有关。