Department of Biological Science, Florida State University, 32306, Tallahassee, FL, USA.
Planta. 1977 Jan;135(3):217-23. doi: 10.1007/BF00384893.
"Killer", a substance extracted from stem tissue of etiolated pea seedlings (Pisum sativum L. v. Alaska), interacts specifically with the far-red-absorbing form of phytochrome (Pfr) in vitro in a temperature-independent, rapid, stoichiometric fashion to cause a loss of phytochrome photoreversibility. The chromatographic, solubility, and spectral properties of partially purified fractions indicate that Killer is a cyclic, unsaturated molecule containing ionizible hydroxyl groups; its molecular weight is unknown, although probably low. Possible mechanisms by which the Killer-phytochrome interaction results in the loss of photoreversibility are discussed.
"Killer",一种从黄化豌豆幼苗(Pisum sativum L. v. Alaska)茎组织中提取的物质,在体外以非温度依赖、快速、化学计量的方式与远红光吸收形式的光敏色素(Pfr)特异性相互作用,导致光敏色素光可逆性丧失。部分纯化级分的色谱、溶解度和光谱性质表明,Killer 是一种含有可电离羟基的环状、不饱和分子;其分子量未知,但可能较低。讨论了 Killer-phytochrome 相互作用导致光可逆性丧失的可能机制。