Department of Biology, Botany Section, Virginia Polytechnic Institute and State University, 24061, Blacksburg, Virginia, USA.
Photosynth Res. 1984 Sep;5(3):251-61. doi: 10.1007/BF00030026.
The initial (in vivo) and total (activity present after preincubation with CO2 and Mg(2+)) activities of ribulose bisphosphate carboxylase were both assayed in extracts of leaves of soybean (Glycine max) plants which had been grown under 4 different irradiance levels. The total carboxylase activity per unit leaf area decreased with decreased irradiance during growth but was not different on a dry weight basis. The initial activity as a percentage of the total activity was unchanged (approximately 95%) except in leaves of plants grown at the lowest irradiance (74%). When the plants grown at the lowest irradiance were exposed to high irradiance, the initial activity was increased to 93% of the total. Light saturated rates of photosynthesis per unit leaf area were lower and saturated at lower irradiance for plants grown at lower irradiances. Initial carboxylase activity was correlated closely (r(2)=0.84) with leaf photosynthesis rate on a dry weight basis.
在不同光照强度下生长的大豆（Glycine max）叶片提取物中，测定了核酮糖二磷酸羧化酶的初始（体内）和总（与 CO2 和 Mg(2+)预孵育后存在的活性）活性。在生长过程中，单位叶面积的总羧化酶活性随光照强度的降低而降低，但按干重计算并无差异。初始活性占总活性的百分比不变（约为 95%），除了在光照强度最低（74%）下生长的叶片中。当光照强度最低的植株暴露在高光强下时，初始活性增加到总活性的 93%。单位叶面积的光饱和光合作用速率较低，在较低的光照强度下达到饱和，对于在较低光照强度下生长的植物而言。初始羧化酶活性与按干重计算的叶片光合作用速率密切相关（r(2)=0.84）。
