Department of Biological Sciences, University of Lancaster, LA1 4YQ, Lancaster, U.K..
Planta. 1973 Jun;114(2):131-42. doi: 10.1007/BF00387471.
A quantitative estimation of the levels of plastidic SDS-extractable polypeptides as separated by polyacrylamide gel electrophoresis is described to demonstrate the practicality of such an approach. Using an internal standard of cytochrome c and expressing all polypeptide levels as cytochrome c relative stain equivalents, the levels of most polypeptides from developing Avena plastids change relative to the period of greening especially over the period 12-20 h. Some changes in certain polypeptides can be shown to be due to plastid senescence rather than plastid development. There is also a distinct difference in the pattern of polypeptides when plastids are isolated from different laminar regions. An incubation study using etioplasts showed of the original 8 polypeptides, six were retained, two were lost, another two were formed during incubation but eleven polypeptides found in the in situ study never appeared.
描述了一种通过聚丙烯酰胺凝胶电泳分离质体 SDS-可提取多肽的水平的定量估计，以证明这种方法的实用性。使用细胞色素 c 的内标，并将所有多肽水平表示为细胞色素 c 的相对染色等价物，来自发育中的燕麦质体的大多数多肽的水平相对于绿化期，特别是在 12-20 小时期间发生变化。某些多肽的某些变化可以归因于质体衰老而不是质体发育。当从不同层区域分离质体时，多肽的模式也存在明显差异。使用黄化质体进行的孵育研究表明，在最初的 8 种多肽中，有 6 种被保留，2 种丢失，另外 2 种在孵育过程中形成，但在原位研究中发现的 11 种多肽从未出现过。
