Institute for Agricultural Research, Tohoku University, 980, Sendai, Japan.
Planta. 1973 Mar;115(1):77-82. doi: 10.1007/BF00388607.
Aggregation of protoplasts of Allium fistulosum L., Brassica chinensis L. and Daucus carota L. was induced in great frequency with gelatin and early products of gelatin degradation. The optimal gelatin concentration was 2-5%. The aggregated protoplasts fused into groups and cell walls were formed around them in culture.
采用明胶和明胶降解早期产物,可大大提高洋葱、白菜和胡萝卜原生质体的聚集频率。最适明胶浓度为 2-5%。培养过程中,聚集的原生质体融合成团,周围形成细胞壁。